Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2011.336.343.Elayarajah RajendranR. Venkatrajah SreekumarSweda SudhakarAsa Janiga SreekumarSoumya 52011145Biomaterial-centred bacterial infections present common and challenging complications with medical implants like ureteral stent which provide substratum for the biofilm formation. Hence the purpose of this study is to make antibacterial stent surface with biodegradable polymer (tocopherol acetate) and anti-infective agents (norfloxacin and metronidazole) using a modified dip-coating procedure. This is done by impregnating the stent pieces in the anti-infective solution (a mixture of norfloxacin-metronidazole and polymer) for uniform surface coating (drug-carrier-coated stents). After coating, agar diffusion test was performed as qualitative test to find out the sensitivity of coated stents against the clinical isolates, Staphylococcus epidermidis and Escherichia coli. Quantitative test was measured by calculating the numbers of adhered bacteria on coated and uncoated stents by incubating the stent pieces in artificial urine. Difference in the number of viable bacteria adhered on the surface of coated and uncoated stents were statistically calculated using chi square test with p<0.05 considered significant. The stent colonising ability of Staphylococcus epidermidis and Escherichia coli in a controlled environment chamber was determined using two-challenge dose of the isolates by in vitro challenge test. In qualitative test, the zone of inhibition around the coated stents showed sensitivity against the clinical isolates. In quantitative test, the number of adhered bacteria on the surface of coated stents was reduced to a significant level (p<0.05). The polymer, tocopherol acetate is highly biodegradable in nature. Due to its degrading ability in body tissues, it releases the anti-infective drugs at a constant and sustained rate.]]>Bayston, R., L.E. Fisher and K. Weber,20093031673173Bayston, R. and S. Barsham,198845235239Bayston, R., N. Grove, T.J. Siegel, D. Lawellin and S. Barsham,1989in vitro by impregnation with antimicrobials.]]>52605609Bharadwaj, R., R. Vidya, B. Dewan and A. Pal,2003in vitro study to evaluate the synergistic activity of norfloxacin and metronidazole.]]>35220226Brody, T.,19992nd Edn.pp: 554-565pp: 554-565Cauda, F., V. Cauda, C. Fiori, B. Onida and E. Garrone,2008in vivo case study.]]>22465472Cerca, N., S. Martinand, S. Sillankorva, K.K. Jefferson, G.B. Pier, R. Oliveira and J. Azeredo,2005Staphylococcus epidermidis and Staphylococcus haemolyticus biofilms.]]>7186778682Danese, P.N.,20029873880Darouiche, R.O.,200435014221429Dollery, C.,19992nd Edn.,pp: 137-140pp: 137-140Elek, S.D. and P.E. Conen,1957Staphylococcus pyogenes for man: A study of the problems of wound infection.]]>38573586Gollwitzer, H., K. Ibrahim, H. Meyer, W. Mittelmeier, R. Busch and A. Stemberger,200351585591Kucers, A., S.M. Crowe and M.L. Grayson,19975th Edn.,pp: 936-938pp: 936-938Kunin, C.M.,19975th Edn.,Jun, L., W. Bochu and W. Yazhou,20062513519Lingeman, J.E., G.M. Preminger, Y. Berger, J.D. Denstedt and L. Goldstone et al.,200316916821688Matl, F.D., A. Obermeier, S. Repmann, W. Friess, A. Stemberger and K.D. Kuehn,20085219571963El-Rehewy, M.S.K., M.A. El-Feky, M.A. Hassan, H.A Abolella, A. Abolyosr, R.M.A. El-Baky and G.F. Gad,2009In vitro efficacy of ureteral catheters impregnated with ciprofloxacin, N-acetylcysteine and their combinations on microbial adherence.]]>318Owlia, P., L. Sadeghzadeh, F. Orang, M. Rafienia and S. Bonakdar,2007Salmonella spp.]]>6597600Pittet, D., D. Tarara and R.P. Wenzel,199427115981601Phromsopha, T. and Y. Baimark,2009b-poly (D, L-lactide) films for controlled release of ibuprofen.]]>4107115Quesada, E.T. and J.K. Light,19931494648Reid, G., S. Sharma, K. Advikolanu, C. Tieszer, R.A. Martin and A.W. Bruce,1994in vitro adhesion and survival of Pseudomonas aeruginosa AK1 on urinary catheters.]]>3814901495Schierholz, J. M. and J. Beuth,2001498793Shaheen, S.M., F.R.S. Ahmed, M.N. Hossen, M. Ahmed, M.S. Amran and M. Anwar-UL-Islam,2006911811191Stickler, D., R. Young, G. Jones, N. Sabbuba and N. Morris,200331306311Surini, S., V. Anggriani and E. Anwar,20099249256Wang, Z.X., B.F. Hong, X. Zhang, W.J. Fu, F.Z. Cui and K. Hu,2010268998