Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2011.882.886Leptadenia pyrotechnica: Comparative Study of Diverse Extracts]]>MoustafaAmal M. Youssef KhodairAhmed I. SalehMahmoud A. 1220111418Comparative acute toxicity studies of the latex and sequential extracts of Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae) were recorded using brine shrimp. The higher toxicities were exhibited in latex; methanol, methanol/dichloromethane (1:1), defatted methanol/dichloromethane (1:1), defatted methanol and dichloromethane extracts. The other extracts; aqueous, alkaloids, ethyl acetate and n-butanol exhibited less toxicities compared with the other extracts. The estimated LC50 and its 95% confidence limits for these extracts expressed in ppm were: methanol, latex 18.84 (11.22-31.61), methanol/dichloromethane 19.95 (7.76-53.70), defatted methanol/dichloromethane 21.38 (7.24-63.10), defatted methanol 28.19 (16.27-48.81) and dichloromethane 30.90 (11.75-79.43). The anti-tumor activities; potato disc assays of methanol, ethyl acetate and alkaloids extracts showed good activities as anti-tumor agent which represented-49.30,-43.20 an -33.60%, respectively. While latex and aqueous extract represented-30.80 and-28.17%, respectively.]]>Abd El-Ghani, M.M. and W.M. Amer,200355607628Anderson, J.E., C.M. Goetz, J.L. McLaughlin and M. Suffness,19912107111Anderson, J.E., M.A. Wenwen, L.D. Smith, C.J. Chang and J.L. McLaughlin,1992Lzndera benzozn. ]]>557183Caprioll, V., G. Cimino, A. de Giulio, A. Madaio, G. Scognamiglio and E. Trivellone,1992103293296Carballo, J.L., Z.L. Hernández-Inda, P. Pérez and M.D. García-Grávalos,2002in vitro cytotoxicity in marine natural products.]]>2002Cioffi, G., R. Sanogo, A. Vassallo, F. Dal Piaz, G. Autore, S. Marzocco and N. De Tommasi,.2006Leptadenia pyrotechnica.]]>69625635El-Hassan A., M. El-Sayed, A.I. Hameda, I.K. Rheeb, A.A. Ahmedc, K.P. Zellerd and R. Verpoorteb,2003Leptadenia arborea.]]>74184187Ferrigni, N.R. and J.L. McLughlin,198447 347352Ho, J.C., C.M. Chen and L.C. Row,2005Tetrapanax papyriferus.]]>6817731775Krishnaraju, A.V., T.V.N. Rao, D. Sundararaju, M. Vanisree, H.S. Tsay and G.V. Subbaraju,2006Artemia salina (Brine Shrimp Test).]]>4115125Mongelli, E., S. Pampuro, J. Coussio, H. Salomon and G. Ciccia,200071145151Moustafa, A.M.Y., A.I. Khodair and M.A. Saleh,2007Leptadenia pyrotechnica.]]>2681697Moustafa, A.M.Y., A.I. Khodair and M.A. Saleh,2009Leptadenia pyrotechnica.]]>47826834Moustafa, A. M.Y., A.I. Khodair and M.A. Saleh,2009Leptadenia pyrotechnica.]]>479941003Noor, F., A. Ahmed, S.M. Imtiazuddin and B. Khan,1993Lepetadenia pyrotechnica.]]>32211212Ohyama, K., L. E. Pelcher, A. Schaefer and L. C. Fowke,1979In vitro binding of Agrobacterium tumefaciens to plant cells from suspension culture.]]>63382387Panwar, J. and J.C. Tarafdar,200665337350Pisutthanan, S., P. Plianbangchang, N. Pisutthanan, S. Ruanruay and O. Muanrit,2004121318Poli, A., M.C. Manca, A. de Giulio, G. Strazzullo, S. de Rosa and B. Nicolaus,2006Lycopersicon esculentum var. San Marzano.]]>69658661Schmidt, D.F.N., R.A. Yunes, E.H. Schaab, A. Malheiros and V.C. Filho et al.,20069200208Moustafa, A.M.Y., A.I. Khodair and M.A. Saleh,2009Leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity.]]>47539552