Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2012.863.870SamarakoonS.A.M.C. BalasuriyaA. RajapakshaR.G.A.S. WickramarachchiW.A.R.T. 1220121518Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modified Cetyltrimethyl Ammonium Bromide (CTAB) method in Gannoruwa. Associated Begomoviruses were detected using Deng 541/Deng 540 and AV 494/AC 1048 primer pairs. TYLCV was detected for the first time in tomato in Sri Lanka using P1V/P4C, TYLCV specific primer pair. Nucleotide sequence of coat protein of isolated TYLCV-GN-SL proved that the Indian strain of ToLC virus was closely related to Tomato Leaf Curl Sri Lanka Virus (TLCV-SL: 97%) and Tomato leaf curl Geminivirus (TLCGV: 93%) through direct sequencing data. TLCV-SL was confirmed as TYLCV isolate. TYLCV was molecularly detected from major tomato growing districts like Badulla, Nuwara-Eliya, Kandy and Matale in Sri Lanka.]]>Anonymous,20042004Ariyarathna, I., T. Liyanage and M. Sagarika,200461328Ber, R., N. Navot, D. Zamir, Y. Antignus, S. Cohen and H. Czosnek,1990112169180Briddon, R.W. and P.G. Markham,199525315320Brown, J.K and M.R. Nelson,1988Chino del tomato virus (CdTV), a whitefly-transmitted geminivirus from Sinaloa, Mexico.]]>72866869Brown, L.G. and G.W. Simone,19941994Cerkauskas, R.F.,20052005Costa, J.M. and E. Heuvelink,20052005pp: 1-20pp: 1-20Czosnek, H. and H. Laterrot,199714213911406De Zoysa, I.J.,19961996pp: 122129Deng, D., P.F. McGrath, D.J. Robinson and B.D. Harrison,1994125327336Fauquet, C.M. and J. Stanley,2003142165189Fauquet, C.M., R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini and X. Zhou,2008Geminivirus strain demarcation and nomenclature.]]>153783821Gafni, Y.,20034915Green, S.K. and G. Kalloo,19941994Koklu, G., A. Rojas and A. Kvarnheden,2006886166Mehrotra, R.S. and A. Aggarwal,20032nd Edn.,pp: 703-735pp: 703-735Melzer, M.J., D.Y. Ogata, S.K. Fukuda, R. Shimabuku, W.B. Borth, D.M. Sether and J.S. Hu,20092009Momol, T., S. Olson, J. Funderburk and R. Sprenkel,19991999Muniyappa, V., H.M. Venkatesh, H.K. Ramappa, R.S. Kulkarni and M. Zeidan et al.,200014515831598Navot, N., M. Zeidan, E. Pichersky, D. Zamir and H. Czosnek,19928211991202Noris, E., A.M. Vaira, P. Caciagli, V. Masenga, B. Gronenborn and G.P. Accotto,1998721005010057Reddy, B.A.,20062006Roye, M.E., M.E. Wernecke, W.A. McLaughlin, M.K. Nakhla and D.P. Maxwell,1999Geminivirus associated with tomatoes and peppers in Jamaica and mixed infection with tomato yellow leaf curl virus.]]>48370378Sawalha, H.,20121612Scholthof, K.B.G., S. Adkins, H. Czosnek, P. Palukaitis and E. Jacquot et al.,201112938954Sugano, J., M. Melzer, A. Pant, T. Radovich, S. Fukuda, S. Migita and J. Uyeda,20112011Tamura, K., J. Dudley, M. Nei and S. Kumar,20072415961599Varma, A. and V.G. Malathi,2003142145164Wyatt, S.D. and J.K. Brown,19968612881293Czosnek, H.,20082008pp: 138-145pp: 138-145Lodhi, M.A., G.N. Ye, N.F. Weeden and B.I. Reisch,199412613