Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1069.1071NajjarA. SaidS. Ben BenaounB. ChetouiC. EzzaouiaM. MradM. Ben 1220131619The study was undertaken in order to evaluate sperm morphology
features of post-thawed semen of Tunisian Arab stallions. Forty two ejaculates
was collected and frozen, during years 2009 and 2010, from 9 stallions aged
between 9 to 24 years. After thawing, sperm morphology was studied after eosin-nigrosin
stain. The percentages of abnormal head, mid piece, flagella, sperm with droplets
and the total abnormal sperm were determined. Analysis of variance was carried
out using SAS software. The results showed that all sperm morphology features
varied among ejaculates within stallion and among stallions (p<0.01). The
percentage of abnormal flagella and total abnormal sperm varied between young
and old stallions (p<0.01). We concluded that freezing and thawing processes
increased abnormal sperm that is due to cell alteration for old and young stallions,
and consequently, the decrease of the quality of the thawed semen of Tunisian
Arab stallions.]]>Bailey, J.L., J.F. Bilaodean and N. Cormier,20002017Blach, E.L, R.P. Amann, R.A. Bowen and D. Frantz,198931283298Brito, L.F.C.,20076249264Chan, S.Y.W., C. Wang, S.T.H. Chan, P.C. Ho, W.W.K. So. Y.F. Chan and H.K. Ma,1989in vitro fertilisation of human oocyte.]]>6142148Christensen, P., J.M. Parlevliet, A. Van Buiten, P. Hyttel and B. Colenbrander,19951769777Garavance, C.G., Z. Champion, I.K.M. Liu and P.J. Casey,199747149155Haras Nationaux,20042004Holt, W.V. and R.D. North,198678447457Jasko, D.J., D.H. Lein and R.H. Foote,1990197389393Katila, T.,2001In vitro evaluation of frozen-thawed stallion semen: A review.]]>42199217Love, C.C., D.D. Varner and J.A. Thompson,20005693100Najjar, A., B. Benaoun, M. Ezzaouia, A.B. Maatoug, M. Magistrini and M.B. Mrad,20108173177Varner, D.D.,200870448462Voss, J.L., B.W. Pickett and E.L. Squires,1981178287289