Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1216.1219Heliotropium crassavicum) Using Stem Explants]]>SatyavaniK. DheepakV. GurudeebanS. RamanathanT. 1220131620Heliotropium crassavicum L. is a sand binder salt marsh
herb with enormous traditional value and widely found in South Asia America
and Europe. In the direct method of regeneration from stem explants, we observed
the maximum number of shoot regeneration after four weeks culture of MS elongation
medium with 2.0 mg L-1 of 2, 4-D (17.27±0.51). It was clear
that MS medium with 2.0 mg mL-1 2, 4-D alone suitable for shoot multiplication
as well as shoot elongation then compared to other combination of auxin and
cytokinin. In vitro shoots were excised from shoot clumps and transferred
to rooting medium containing 2, 4-dichlorophenoxy acetic acid (0.5-3.0 mg L-1).
The maximum number of root regeneration (6.4±0.416) and root length (6.08±0.07)
were observed in MS rooting medium fortified with 2.5 mg L-1 of 2,
4-D after 2 weeks of culture. 85% of in vitro raised plantlets with well-developed
shoots and roots were transferred to ex vivo conditions into polythene
bag containing sterile compost with ratio (v/v/v) of organic fertilizer: sand:
peat (1: 2: 2; 3: 1:0 or 2: 2: 1). Sixty five percent of acclimated plants were
transferred to the pots under full sun where they grew well without any detectable
phenotypic variations.]]>Cavusoglu, A., Z. Ipekci-Altas, K. Bajrovic, N. Gozukirmizi and A. Zehir,2011Populus deltoids Bartram ex Marsh. with tissue culture.]]>1032163221Afrobz, F., S. Hassan, L.S. Bari, R. Sultana and N. Begum et al.,2009In vitro shoot proliferation and plant regeneration of Physalis minima L. a perennial medicinal herb.]]>44453456.Gurudeeban, S., K. Satyavani, T. Ramanathan and T. Balasubramanian,2012Ruta graveolens.]]>1114971504.Jeyachandran, R., X. Baskaran and L. Cindrella,2010In vitro direct regeneration of nodal explant of Justicia prostrate Gamble.]]>19093Kirtikar, K.R. and B.D. Basu,19982nd Edn.,pp: 804-806pp: 804-806McAuley, M.,2007Murashige, T. and F. Skoog,196215473497Ramanathan, T., K. Satyavani and S. Gurudeeban,2011In vitro plantlet regeneration of gum-bearing tree Aegle marmelos.]]>34750Satyavani, K., T. Ramanathan and S. Gurudeeban,2011Citrullus colocynthis) from stem explant.]]>3246253Sharma, R.A., B. Singh, D. Singh and P. Chandrawat,2009311531175Wolff, K.C.F.,19773rd Edn.,