Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.127.134Schisandra chinensis (Turcz.) Baillon]]>SunDan LangWen-Xiang LiHong-Bo GuoXiu-Wu LianMei-Lan PiaoZhong-Yun 32013163A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L-1 TDZ and 0.2 mg L-1 Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.]]>Bates, S., J.E. Preece, N.E. Navarrete, J.W.V. Sambeek and G.R. Gaffney,1992Fraxinus americana L).]]>312129Bonneau, L., N. Beranger-Novat and J. Monin,1994Euonymus europaeus L.).]]>13135138Bornman, C.H.,19931993pp: 246-260pp: 246-260Capelle, S.C., D.W.S. Mok, S.C. Kirchner and M.C. Mok,19836-( Δ2-isopentenyl) [8-14C] adenosine in callus tissues of Phaseolus lunatus L.]]>73796802Chen, A.H., J.L. Yang, Y. da Niu, C.P. Yang, G.F. Liu, C.Y. Yu and C.H. Li,20103 and BA on somatic embryo development.]]>102357364Das, P., S. Samantray, A.V. Roberts and G.R. Rout,1997In vitro somatic embryogenesis of Dalbergia sissoo Roxb.-a multipurpose timber-yielding tree.]]>16578582Fei, S.Z., T. Riordan and P. Read,200270275279Fiola, J.A., M.A. Hassan, H.J. Swartz, R.H. Bors and R. McNicols,199020223228Gairi, A. and A. Rashid,2004762933Goussard, P.G. and J. Wiid,1992in vitro somatic embryogenesis combined with heat therapy.]]>138183Hancke, J.L., R.A. Burgos and F. Ahumada,1999Schisandra chinensis (Turcz.) Baill.]]>70451471Harshavardhan, D., T.S. Rani, K. Ulaganathan and N. Seetharama,200219163171Hikino, H. and Y. Kiso,1988Vol. 2.pp: 39pp: 39Huetteman, C.A. and J.E. Preece,199333105119Kartha, K.K.,19871987pp: 447-458pp: 447-458Kim, T.D., V.R. Anbazhagan and J.I. Park,2005Schisandra chinensis (TURCZ.) Baill.]]>41253257Lin, C.S., C.C. Lin and W.C. Chang,2004Bambusa edulis.]]>767582Liu, J. and P.G. Xiao,19948445451Lelu, M.A., C. Bastien, K. Klimaszewska, P.J. Charest,1994Larix x leptoeuropaea): Part 2. Control of germination and plantlet development.]]>36117127Litz, R.E. and D.J. Gray,199511416425Maluszynski, M., B.S. Ahloowalia and B. Sigurbjornsson,1995in vivo and in vitro mutation techniques for crop improvement.]]>85303315Matsuta, N. and T. Hirabayashi,1989Vitis vinifera L.).]]>7684687Merkle, S.A. and C.J. Nairn,200541602619Murthy, B.N.S., S.J. Murch and P.K. Saxena,1995A. hypogaea L.): Endogenous growth regulator levels and significasnce of cotyledons.]]>94268276Murthy, B.N.S., J. Victor, R.P. Singh, R.A. Fletcher and P.K. Saxena,1996In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron.]]>19233240Neuman, M.C., J.E. Preece, G. Gaffney and J.W. Van Sambeek,1988Juglans nigra L.).]]>23807810Neuman, M.C., J.E. Preece, J.W. Van Sambeek and G.R. Gaffney,199332918Ohtaki, Y., T. Hida, K. Hiramatsu, M. Kanitani and T. Ohshima et al.,1996Schizandra fruits.]]>16751755Paiva, M. and J. Janick,1983In vivo and in vitro production of alkaloids in Theobroma cacao L.]]>131249273Preece, J.E. and S. Bates,19901990Price, H.J., R.H. Smith,1979Gossypium Klotzschienum andress.]]>145305307Redenbaugh, K., P. Viss, D. Slade and J.A. Fujii,19863473493Smiskova, A., H. Vlasinova and L. Havel,2005Schisandra chinensis.]]>49451454Saunders, R.M.K.,2000Pages: 146Pages: 146Trolinder, N.L. and C. Xhixian,19898133136Wang, H.C., J.T. Chen, S.P. Wu, M.C. Lin and W.C. Chang,2003Areca catechu L. (Arecaceae).]]>393436Zhang, B.H., R. Feng, F. Liu and O. Wang,200142916Zhou, J.Y., H. Ma, F.X. Guo and X. Luo,1994Cayratia japonica.]]>367379Ohishi, N., Y. Sakamoro and T. Hirosawa,199439137145Murashige, T. and F. Skoog,196215473497