Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1449.1458Fasciola gigantica using Paramyosin Antigen as a Candidate for Vaccine Production]]>Abou-ElhakamH. RabeeI. EL DeebS. EL AmirA. 1220131622Yet no vaccine to protect ruminants against liver fluke infection
has been commercialized. In an attempt to develop a suitable vaccine against
Fasciola gigantica (F. gigantica) infection in rabbits, using 97
kDa Pmy antigen. It was found that, the mean worm burdens and bile egg count
after challenge were reduced significantly by 58.40 and 61.40%, respectively.
On the other hand, immunization of rabbits with Pmy induced a significant expression
of humoral antibodies (IgM, total IgG, IgG1, IgG2 and IgG4) and different cytokines
(IL-6, IL-10, IL-12 and TNF-α). Among Ig isotypes, IgG2 and IgG4 were most
dominant Post-infection (PI) while, recording a low IgG1 level. The dominance
of IgG2 and IgG4 suggested late T helper1 (Th1) involvement in rabbits
cellular response. While, the low IgG1 level suggested Th2 response to adult
F. gigantica worm Pmy. Among all cytokines, IL-10 was the highest in
rabbits immunized with Pmy PI suggesting also the enhancement of Th2 response.
It was clear that the native F. gigantica Pmy is considered as a relevant
candidate for vaccination against fascioliasis. Also, these data suggested the
immunoprophylactic effect of the native F. gigantica Pmy which is mediated
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