Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.726.730Fasciola sp., of Cattle in Qena, Upper Egypt]]>OmarMosaab A. MetwallyAsmaa M. SultanKhaled 1220131615The species of liver fluke of the genus Fasciola (phylum platyhelminthes, order Digenea, Family Fasciolidae) are obligatory parasites that inhabit the large biliary ducts of herbivore animals as well as man. Reports on the species of Fasciola present in the Nile Delta, Egypt, appear controversial. In the current study a precise identification of Fasciola isolates from cattle in Qena province, Upper Egypt was done based on examination of the second Internal Transcribed Spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI). Amplification, sequencing and phylogenetic examination revealed that the collected Fasciola isolates represent only one species which is Fasciola hepatica.]]>Adlard, R.D., S.C. Barker, D. Blair and T.H. Cribb,199323423425Amer, S., Y. Dar, M. Ichikawa, Y. Fukuda, C. Tada, T. Itagaki and Y. Nakai,2011Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers.]]>60512Bowles, J., D. Blair and D.P. McManus,19954103109Dar, Y., S. Amer, A. Mercier, B. Courtioux and G. Dreyfuss,2012Fasciola spp. (Digenea: Fasciolidae) in Egypt.]]>19177182El-Rahimy, H.H., A.M. Mahgoub, N.S. El-Gebaly, W. M. Mousa and A.S. Antably,2012Fasciola species potentially causing zoonotic disease in Egypt.]]>11111031111Garey, J.R. and D.R. Wolstenholme,198928374387Higgins, D.G. and P.M. Sharp,198873237244Huang, W.Y., B. He, C.R. Wang and X.Q. Zhu,2004Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence.]]>1207583Itagaki, T. and K. Tsutsumi,1998Fasciola in Japan: Genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA.]]>28777781Leon-Regagnon, V., D.R. Brooks and G.P.P. de Leon,1999Haematoloechus Looss, 1899 (Digenea: Plagiorchiformes): Molecular and morphological evidence.]]>85935946Lotfy, W.M. and G.V. Hillyer,2003Fasciola species in Egypt.]]>6922Mas-Coma, S.,20042004pp: 305-322pp: 305-322Morgan, J.A. and D. Blair,1995Echinostoma: An aid to establishing relationships within the 37-collar spine group.]]>111609615Prasad, P.K., V. Tandon, A. Chatterjee and S. Bandyopadhyay,2007Fasciolopsis buski (Lankester, 1857) Looss, 1899.]]>10115811587Tamura, K., J. Dudley, M. Nei and S. Kumar,20072415961599White, B.A.,1993Vol. 15,Pages: 392Pages: 392