Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.901.910Phaseolus vulgaris L.) Ecotype Fagiolo
Occhio Nero Di Oliveto Citra Using
Agronomic, Biochemical and Molecular Approaches]]>ZaccardelliMassimo PentangeloAlfonso TripodiPasquale 1220131618Common bean (Phaseolus vulgaris) is the most important
grain legume and plays a significant role in human nutrition being a major source
of dietary protein and representing a rich source of minerals and certain vitamins.
Several large germplasm collections have been established, which contain large
amounts of genetic diversity, including wild and domesticated species. In this
study agronomic, biochemical and molecular characterization of landrace bean
named Fagiolo occhio nero di
Oliveto Citra (Phaseolus
vulgaris L.), is described. Seeds were obtained by local farmers and field
trials were carried out during years 2009-2010, in the typical cultivation site
(Oliveto Citra, Salerno Province), using two different densities of investment.
During 2011, in order to evaluate the performance in different environments,
field trials were conducted in three localities (Battipaglia, Oliveto Citra
and Controne). Data analysis shows good adaptability across locations and similar
grain yield using two spacings
of seeds. Morphological characterization and molecular analysis, using AFLP
and Minisatellite molecular markers, were performed on ten biotypes
collected from local farmers. Seeds characterization showed variability on the
violet area surrounding the hilum (named as eye) while markers have provided
useful information on relationships between biotypes. Biochemical analysis,
which includes the contents of protein, minerals and antioxidants, shows how
the composition is consistent with respect to other landraces and commercial
cultivars. The landrace under study revealed genetic stability and good adaptation
to cultivated environment with best performance in the native area. In addition,
the bio-agronomic characteristics are in accord with studies reported in literature.]]>AOAC.,199916th Edn.,Blair, M.W., G. Iriarte and S. Beebe,2006Phaseolus vulgaris L.) cross.]]>11211491163Bodwell, C.E., L.D. Satterlee and L.R. Hackler,1980in vitro enzymic digestion methods.]]>33677686Caldas, V.G. and M.W. Blair,2009Phaseolus vulgaris L.).]]>119131142Cardador-Martinez, A., E. Castano-Tostado and G. Loarca-Pina,2002Phaseolus vulgaris) against aflatoxin B1.]]>196269Casquero, P.A., M. Lema, M. Santalla and A.M. de Ron,20065310211032Dinelli, G., A. Bonetti, M. Minelli, I. Marotti, P. Catizone and A. Mazzanti,2006Phaseolus vulgaris L.) ecotypes.]]>99105114Doyle, J.,19911991pp: 283-293pp: 283-293Escribano, M.R., A.M. de Ron and J.M. Amurrio,19947616Espinosa-Alonso, L.G., A. Lygin, J.M. Widholm, M.E. Valverde and O. Paredes-Lopez,2006Phaseolus vulgaris L.).]]>5444364444Falconer, D.S. and T.F.C. Mackay,19964th Edn.,Pages: 464Pages: 464Foschiani, A., F. Miceli and M. Vischi,2009Phaseolus vulgaris L.) accessions at phenotype and molecular level: A preliminary approach.]]>56445453Gepts, P., T.C. Osborn, K. Rashka and F.A. Bliss,1986Phaseolus vulgaris L.): Evidence for multiple centers of domestication.]]>40451468Gepts, P., F. Aragao, E. de Barros, M.W. Blair and R. Brondani et al.,2008Phaseolus Beans, a Major Source of Dietary Protein and Micronutrients in the Tropics.]]>2008pp: 113-143pp: 113-143Hawtin, G., M. Iwanaga and T. Hodgkin,19971997pp: 277-288pp: 277-288Janssen, P., R. Coopman, G. Huys, J. Swings and M. Bleeker et al.,199614218811893Jeffreys, A.J.,20051110351039Limongelli, G., G. Laghetti, P. Perrino and A.R. Piergiovanni,1996Phaseolus vulgaris L.) from Basilicata, Southern Italy.]]>92393399McCouch, S.,20042e347e347Moraghan, J.T. and K. Grafton,199774251256Paredes, M.C., V.V. Becerra and J.U. Tay,2009Phaseolus vulgaris L.) genotypes race chile.]]>69486495Perazzini, R., D. Leonardi, S. Ruggeri, D. Alesiani, G. D'Arcangelo and A. Canini,2008Phaseolus vulgaris L. landraces cultivated in Central Italy.]]>63211218Piergiovanni, A.R. and L. Lioi,20102837862Pinheiro, C., J.P. Baeta, A.M. Pereira, H. Domingues and C.P. Ricardo,2010Phaseolus vulgaris L. germplasm.]]>23319325Price, M.L., S. van Scoyoc and L.G. Butler,19782612141218Radojevic, M. and V.N. Bashkin,20062nd Edn.,pp: 443-447pp: 443-447SAS Institute Inc.,2007Shellie-Dessert, K.C. and F.A. Bliss,19911991pp: 649-671pp: 649-671Sneath, P.H.A. and R.R. Sokal,1973pp: 573pp: 573Vauterin, L. and P. Vauterin,19921992pp: 1922Vos, P., R. Hogers, M. Bleeker, M. Reijans and T. van de Lee et al.,19952344074414Yanez, E., Y. Zacarias, M. Aguayo, M. Vasquez and E. Guzman,1995Phaseolus vulgaris) released in Chile.]]>47301307Zaccardelli, M., P. Ceroni and U. Mazzucchi,1999Xanthomonas arboricola pv. pruni.]]>81173179Zaccardelli, M., R. Torre, F. Campanile and I. Giordano,2004Phaseolus vulgaris L.) ecotype Fagiolo occhio nero di Oliveto Citra.]]>2004Zeven, A.C.,1998104127139