Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.1237.1241in vivo Study]]>Al-RubaeiZ.M. Malik MohammadTaghreed U. AliLayla Karim 1220141712Plants have been one of the important sources of medicine
even since the dawn of human civilization. Curcumin has been found to possess
tremendous therapeutic potency as antiinflammatory, antioxidant and antimicrobial
agent. The present study was designed to examine possible potential therapeutic
and protective effect of curcumin from oxidative stress and on total antioxidant
capacity in liver damage. The study was conducted using H2O2
as inducing agent of oxidative stress in vivo. Rats were randomly divided
into five groups, where n = 20 for each group. Group 1 (G1) rats served as control
group. Group 2 (G2) rats subjected to experimentally induced oxidative stress
by the ad libitum supply of drinking water containing 0.5% H2O2(v/v)
was prepared daily over entire 60 days. Group 3 (G3) rats received H2O2
for sixty days followed by giving 200 mg kg-1 of curcumin for 30
days. Group 4 (G4) was simultaneously given curcumin (200 mg kg-1)
for 15 days then followed by receiving H2O2 with curcumin
for sixty days. Group 5 (G5) rats was received H2O2 for
sixty days followed by giving 200 mg kg-1 of N-acetyl 1-cystine as
standard drug for 30 days. Levels of marker enzymes (ALT, AST and ALP), uric
acid, Total Protein (TP) and tumor necrosis factor (α-TNF) were assessed
in serum for all studied groups. Malondialdehyde (MDA), 8-hydroxy-2-deoxyguinosine,
Total Antioxidant Capacity (TAC), reduced glutathione (GSH), superoxide dismutase
(SOD), catalase (CAT) were assayed in liver homogenates for all studied groups.The
results revealed significant increase (p<0.05) in levels of ALT, AST, ALP,
uric acid and α-TNF while there are significant decrease (p<0.05) in
levels of TP in G2 comparing to G1. Also there are significant differences (p<0.05)
between G3 and G4 comparing to G2 and between G3, G4 and G5 which curcumin elicited
a significant hepatoprotective activity by lowering the levels of serum marker
enzymes and lipid peroxidation. The results also revealed a significant increase
(p<0.05) in levels of MDA and 8-H-2-deoxy guinosine while there was significant
reduction (p<0.05) in TAC, GSH, SOD and catalase in G2 comparing to G1. Also
there are significant differences (p<0.05) between G3 and G4 comparing to
G2 and between G3, G4 and G5. The conclusion could be drown from this study
that the ability of curcumin as therapeutic agent and hepatoprotective against
liver damage from oxidative damage and on TAC more than N-acetyl 1-cystine related
to its antioxidant and free radical scavenger activity.]]>Rani, M.S., S. Emmanuel, M.R. Sreekanth and S. Ignacimuthu,2010in vivo antioxidant and hepatoprotective activity of Cassia occidentalis Linn. against paracetamol induced liver toxicity in rats.]]>26770Akinrinmade, J.F. and A.S. Akinrinde,2013Ocimum gratissimum on intestinal ischemia-reperfusion injury in rats.]]>3591602Zhou, T., R. Zong, Z. Zhang, C. Zhu and F. Pan et al.,20125350335043Choi, K., J. Kim, G.W. Kim and C. Choi,20096213222Jain, S., S. Shrivastava, S. Nayak and S. Sumbhate,2007Curcuma longa Linn.]]>1119128Tushar, S. Basak, G.C. Sarma and L. Rangan,2010132286296Hamid, A., S.B. Budin, R.A.P. Mohamed, N.A. Manaf and N.Y. Yuhana et al.,2011zerumbet smith ethyl-acetate extract against paracetamol-induced hepatotoxicity in Sprague-dawley rats.]]>515191525Rao, M.N.,1996164446Cekmen, M., Y.O. Ilbey, E. Ozbek, A. Simsek, A. Somay and C. Ersoz,20094714801484Fossati, P., L. Prencipe and G. Berti,198026227231Reitman, S. and S. Frankel,1957285663Kind, P.R.N. and E.J. King,19547322326Buege, J.A. and S.D. Aust,19781978pp: 302-310pp: 302-310Miller, N.J., C. Rice-Evans, M.J. Davies, V. Gopinathan and A. Milner,199384407412Ellman, G.L.,1959827077Sun, Y., L.W. Oberley and Y. Li,198834497500Aebi, H.,19821982Al-Shammry, Z.M.M. and B.N. Al-Okaily,2009Vitis vinifera L.) on hepaticfunction and damage induced by hydrogen peroxide and methionineoverload in rats.]]>21935Chuang, S.E., A.L. Cheng, J.K. Lin and M.L. Kuo,200038991995Aniya, Y., T. Koyama, C. Miyagi, M. Miyahira, C. Inomata, S. Kinoshita and T. Ichiba,2005Crassocephalum crepidioides from the Okinawa Islands.]]>281923Vrca, V.B., F. Skreb, I. Cepelak and L. Mayer,2004547989Ogbera, A.O. and A.O. Azenabor,20102010Farghaly, H.S. and M.A. Hussein,2010442664274Min, K.S., T. Horie, N. Tetsutchikawahara and S. Onosaka,2005in vitro.]]>51497503Al-Aubaidy, H.A. and H.F. Jelinek,2011164899904Savu, O., C. Ionescu-Tirgoviste, V. Atanasiu, L. Gaman, R. Papacocea and I. Stoian,201240709716Valkonen, M. and T. Kuusi,199738823833Ramani, G., G. Kavitha, P.K. Dhass and R. Aruna,20112329332Yanadaiah, J.P., S.M. Lakshmi, K.N. Jayaveera and Y. Sudhakar,20113502506Loki, A.L. and T. Rajamohan,200340354357