Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.254.259WangLiang XiangweiFu ZengYan HouYunpeng ZhuShien 22014172Cryopreserved oocytes show low developmental ability. To understand the mechanism underlying their development impairment, study was designed to determine the effect of epinephrine on the in vitro developmental competence of vitrified mouse oocytes. Mature oocytes were vitrified using Open Pulled Straw (OPS) method. The vitrified oocytes were warmed and introduced into M2 medium which contains epinephrine at different concentrations (10-2, 10-4, 10-6, 10-8 mol L-1) in an incubator for 1 h. Then the survival rate of the oocytes was evaluated and the subsequent development of oocytes was assessed through in vitro Fertilization (IVF). Furthermore, the levels of intracellular ROS, GSH and the concentration of ATP were determined among 10-4 mol L-1 epinephrine-treated group, vitrification group and fresh group. Results showed that vitrified oocytes treated with 10-4) mol L-1 epinephrine had significant higher rates of cleavage (66.4 vs.45.2%) and blastocyst (47.2 vs. 34.7%) than no epinephrine treated group, as well as more blastocyst cells (54.5 vs. 36.8) and lower ratio of apoptotic cells (5.9 vs. 21.5%; p<0.05). Further experiment found that 10-4 mol L-1 epinephrine treatment could significantly reduce intracellular ROS level and enhance cytoplasmic ATP concentration (p<0.05), but there was no different in GSH level compared to vitrification group. In conclusion, epinephrine could promote vitrified oocytes cryosurvival and their subsequent development ability, which maybe related with the changes of intracellular ROS level and ATP content.]]>Ahn, H.J., I.P. Sohn, H.C. Kwon, D.H. Jo, Y.D. Park and C.K. Min,200261466476Anitole-Misleh, K.G. and K.M. Brown,20041373950Cikos, S., P. Rehak, S. Czikkova, J. Vesela and J. Koppel,200713311391147Cikos, S., J. Vesela, G. Il'kova, P. Rehak, S. Czikkova and J. Koppel,200571145153Costa, V.M., R. Silva, L.C. Tavares, R. Vitorino and F. Amado et al.,20092608496Favetta, L.A., E.J.S. John, W.A King and D.H. Betts,2007shc and intracellular ROS in permanently arrested early embryos. ]]>4212011210Gupta, M.K., S.J. Uhm and H.T. Lee,2010in vitro development of oocytes vitrified before or after in vitro fertilization.]]>9326022607Halliwell, B.,19925916091623Hauser, S.L. and D.L. Longo,20062006pp: 427-457pp: 427-457Herlenius, E. and H. Lagercrantz,2001652137Huang, R., G. Zhuang, and Q. Liu,2003236264Katayama, K.P., J. Stehlik, M. Kuwayama, O. Kato and E. Stehlik,200380223224Kim, M.O., S.I. Na, M.Y. Lee, J.S. Heo and H.J. Han,200810414071420Manipalviratn, S., Z.B. Tong, B. Stegmann, E. Widra, J. Carter and A. DeCherney,20119518391841Milosevic, J., S.C. Schwarz, K. Krohn, M. Poppe, A. Storch and J. Schwarz,200592718729Pereira, R.M. and C.C. Marques,20089262277Sakatani, M., K. Yamanaka, S. Kobayashi and M. Takahashi,2008in vitro early stage bovine embryos.]]>54496501Salvetti, P., S. Buff, M. Afanassieff, N. Daniel, P. Guerin and T. Joly,201074847855Shimizu, T., Y. Nakanishi, M. Nakahara, N. Wada and Y. Moro-Oka et al.,2010in vitro.]]>47181190Somfai, T., M. Ozawa, J. Noguchi, H. Kaneko and N.W. Kuriani Karja et al.,2007in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: Effect of cryopreservation on oocyte antioxidative system and cell cycle stage.]]>55115126Sun, Q.Y., G.M. Wu, L. Lai, K.W. Park and R. Cabot et al.,2001in vitro.]]>122155163Takahashi, T., E. Takahashi, H. Igarashi, N. Tezuka and H. Kurachi,200366143152Trounson, A. and C. Kirby,198952778786Van Blerkom, J. and M.N. Runner,1984171335355Van Blerkom, J. and P.W. Davis,199427165193Van Blerkom, J., P.W. Davis and J. Lee,1995in-vitro fertilization and embryo transfer.]]>10415424Wang, L., J. Liu, G.B. Zhou, Y.P. Hou, J.J. Li and S.E. Zhu,201185884894Yan, C.L., X.W. Fu, G.B. Zhou, X.M. Zhao, L. Suo and S.E. Zhu,201093959966Yoneda, A., K. Suzuki, T. Mori, J. Ueda and T. Watanabe,2004in vitro development of porcine embryos.]]>50287295Zhang, X., X.Q. Wu, S. Lu, Y.L. Guo and X. Ma,200616841850Zhao, X.M., W.H. Du, D. Wang, H.S. Hao, Y. Liu, T. Qin and H.B. Zhu,2011in vitro culture.]]>78942950Zhou, G.B. Y.P. Hou, J.F. Yang, Q.E. Yang and Z.Q. Quan et al.,2005160153163