Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.735.739Trichoderma reesei Cef19 and its
Application in the Production of Bio-ethanol]]>KumarM. Ravi KumaranM.D. Bala BalashanmugamP. RebeccaA. Immaculate Nancy KumarD.J. Mukesh KalaichelvanP.T. 52014175The present study aimed at the production of cellulase enzyme
from the cellulolytic fungi Trichoderma reesei CEF19 and subsequent application
of the cellulase for the fermentation of ethanol. For the same, the cellulolytic
fungi, Trichoderma reesei CEF19 was isolated and was allowed to produce
cellulase enzyme using optimized conditions. The cellulase enzyme was extracted
and purified with the help of ammonium sulphate precipitation, dialysis followed
by ion exchange chromatography with DEAE-Sephadex column. The purified cellulase
enzyme was characterized using SDS-PAGE analysis. The saccharification of the
cellulosic substrates was done using the cellulase enzyme. The fermentation
of saccharified cellulosic substrates into ethanol was carried out using Saccharomyces
cerevisiae. From the results obtained, rice straw was found to be the better
source for the ethanol production when compared to the other substrates.]]>Aneja, K.R.,20054th Edn.,Pages: 607.Pages: 607.Chandel, A.K., E.S. Chan, R. Rudravaram, M.L. Narasu, L.V. Rao and P. Ravindra,200721432Balan, V., L.C. Sousa, S.P.S. Chundawat, D. Marshall, L.N. Sharma, C.K. Chambliss and B.E. Dale,2009Populus nigra).]]>25365375Pasha, C., B.C. Sekhar, B. Srinivas, K. Balakrishna and N. Hanumalal,201272616620Devi, M.C. and M.S. Kumar,2012311451152Li, D.C., M. Lu, Y.L. Li and J. Lu,2003Chaetomium thermophilum CT2.]]>33932937Eriksen, J. and J. Goksoyr,1977Chaetomium thermophile var. dissitum.]]>77445450Foody, B.E. and K.J. Foody,19911991pp: 1225pp: 1225Ganju, R.K., S.K. Murthy and P.J. Vithayathil,1990Chaetomium thermophile var. coprophile.]]>197245255Jahangeer, S., N. Khan, S. Jahangeer, M.S. Shahzad, S. Ahmad, A. Ahmed and S. Khan,200537739748Khan, J.A. and S.K. Singh,20111179187Khandke, K.M., P.J. Vithayathil and S.K. Murthy,1989Thermoascus aurantiacus.]]>274491500Laemmli, U.K.,1970227680685Kumari, B.L., M. Hanumasri and P. Sudhakar,2011119Liu, C.L., J.Z. Wen and M.X. Yang,200738101106Nipa, M.N., S. Sultana and M.A. Hakim,2006Aspergillus humicola.]]>23174176Roslan, A.M., P.L. Yee, U.K.M. Shah, S.A. Aziz and M.A. Hassan,2011Aspergillus sp.]]>6188193Schubert, C.,200624777784Shimoyama, M., M. Kaneko, K. Yoshida, K. Imou and A. Osaki,201190182186Tong, C.C., A.L. Cole and M.G. Shepherd,1980Thermoascus aurantiacus.]]>1918394Zhu, J.Y. and X.J. Pan,201010149925002