Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.931.936in vitro]]>SafariManouchehr GhahariLaya Babak Hossein Zadeh ZoroufchiM.D. 72014177Some growth factors, such as Epidermal Growth Factor (EGF), Growth Hormone (GH) and Platelet Derived Growth Factor (PDGF) have beneficial effects on keratinocyte proliferation and wound healing. Although the mechanism of these factors is unclear. In response to injury, growth factors are secreted by kinds of cutaneous cells. The goal of this project is to investigate the factors that could cause proliferate of the keratinocyte cells in vitro. The keratinocytes were removed from rat pups (10 days). Cultured in media with different concentration of GH, PDGF and EGF separately. The proliferation of cells was evaluated by the method of MTT and 3H-thymidine incorporation. Proliferation of keratinocytes was significantly higher in experimental groups than in control group. EGF maximally stimulated at 10 and 25 ng mL-1. PDGF-BB maximally stimulated at 50 ng mL-1, respectively. And maximal stimulation of GH was 2.5 IU L-1. GH, PDGF-BB and EGF stimulate keratinocyte cells proliferation in different concentration. These growth factors could play in healing of the skin.]]>Ansel, J.C., J.P. Tiesman, J.E. Olerud, J.G. Krueger and J.F. Krane et al.,199392671678Bevan, S., B. Woodward, R.L. Ng, C. Green and R. Martin,199723525532Burling, K., M.A. Seguin, P. Marsh, K. Brinkman and J. Madigan et al.,20006111501155Cook, J.R., M.K. Mody and M.E. Fini,19994031223131Denk, P.O., J. Hoppe, V. Hoppe and M. Knorr,2003273544Denk, P.O. and M. Knorr,2002991519Haber, M., Z. Cao, N. Panjwani, D. Bedenice, W.W. Li and P.J. Provost,20036211217Heldin, C.H. and B. Westermark,1999in vivo role of platelet-derived growth factor.]]>7912831316Hongo, M., M. Itoi, N. Yamaguchi and J. Imanishi,199254916Hongo, M., M. Itoi, Y. Yamamura, N. Yamaguchi and J. Imanishi,199334401404Hoppenreijs, V.P., E. Pels, G.F. Vrensen, P.C. Felten and W.F. Treffers,199334637649Imanishi, J., K. Kamiyama, I. Iguchi, M. Kita, C. Sotozono and S. Kinoshita,200019113129Jorgensen, P.H. and H. Oxlund,199644047Jost, M., C. kari and U. Rodeck,200010505510Knorr, M., M. Volker, P.O. Denk, K. Wunderlich and H.J. Thiel,1997235667671Lal, S.O., S.E. Wolf and D.N. Herndon,200010S39S43Lee, S.W., S.H. Kim, J.Y. Kim and Y. Lee,201063e364e369Lederle, W., N. Linde, J. Heusel, J. Bzyl and E.C. Woenne et al.,2010176981994Li, H., X. Fu, L. Zhang, Q. Huang, Z. Wu and T. Sun,20081454148Marikovsky, M., P. Vogt, E. Eriksson, J.S. Rubin, W.G. Taylor, S. Joachim and M. Klagsbrun,1996106616621Pancholi, S., A. Tullo, A. Khaliq, D. Foreman and M. Boulton,199823618Pastore, S., F. Mascia, V. Mariani and G. Girolomoni,200812813651374Pell, J.M. and P.C. Bates,199213019421950Penha-Goncalves, M.N., D.E. Onions and L. Nicolson,19977375378Rasmussen, L.H., C. Garbarsch, D. Schuppan, D. Moe, K. Horslev-Pedersen, F. Gottrup and H. Steenfos,199423136Reuterdahl, C., C. Sundberg, K. Rubin, K. Funa and B. Gerdin,19939120652075Rollman, O., U.B. Jensen, A. Ostman, L. Blound, S.M. Gustafsdottir and T.G. Jensen,2003120742749Sun, X.Q., X.B. Fu, W. Chen and T.Z. Sun,2004161318Shin, J.U., J.H. Park, B.C.Cho and J.H. Lee,2012225135140Vavken, P., F.A. Saad and M.M. Murray,20102811071112Wieman, T.J., J.M. Smiell and Y. Su,199821822827Wong, V.W., B. Levi, J. Rajadas, M.T. Longaker and G.C. Gurtner,20122012Wu, N.L., Y.C. Chiang, C.C. Huang, J.Y. Fang, D.F. Chen and C.F. Hung,201019e173e181Xian, W., M.P. Rosenberg and J. DiGiovanni,19971414351444Yan, F., Y.N. Hui, Y.J. Li, C.M. Guo and H. Meng,2007221244250