Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2016.122.135KundaRony Marsyal HandayaniNiken Satuti Nur WijayantoHery WidayantiRini 32016193Cuscuses is marsupials animal (Phalangeridae) which has limited spread in eastern Indonesia (Sulawesi, Maluku, Papua and Timor islands), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscuses under captivating condition is an alternative solution to protect from extinction. This study aimed to determine nucleotide sequences and genetic marker on cyt b gene with sequencing method of each species on two provinces. Whole genome DNA was extracted from 22 samples of cuscuses obtained from different habitats, Maluku (13 individuals) and Papua (8 individuals) according to the protocol of Qiamp DNA Blood Mini Kit (Qiagen) and then it was used as template for amplification of cyt b gene by using PCR method. The PCR product were then purified using column chromatography and were used as template for sequencing reaction. Results sequencing of cyt b gene were analyzed using MEGA program versions 6.0. The PCR product gives results nucleotides of 982 bp according to database GeneBank and sequencing product gives results nucleotides of 771 bp. Nucleotides alignment of Phalanger members was found 24 nucleotides distinguishing and Spilocuscus members was found 11 nucleotides distinguishing, which can be used as genetic marker between Phalanger and Spilocuscus members from Papua and Maluku.]]>Aplin, K. and K. Helgen,2008Spilocuscus wilsoni.]]>2008Arnason, U., J.A. Adegoke, K. Bodin, E.W. Born and Y.B. Esa et al.,20029981518156Baillie, J. and B. Groombridge,19961996Brum, G., L.K. McKane and G. Karp,19942nd Edn.,Pages: 410Pages: 410Crosby, K., M. Bassarova, M. Archer and K. Carbery,20042004pp: 161-176pp: 161-176Crosby, K.,2007Strigocuscus (Marsupialia, Phalangeridae), with description of a new genus and three new species.]]>313358Crosby, K. and C.A. Norris,2003Strigocuscus celebensis and Wyulda squamicaudata (Diprotodontia, Phalangeridae) and a revised diagnosis of the tribe Trichosurini.]]>3414114Irwin, D.M., T.D. Kocher and A.C. Wilson,1991b gene of mammals.]]>32128144Darlington, P.J.,1966Pages: 675Pages: 675Flannery, T., M. Archer and G. Maynes,19871987pp: 477-506pp: 477-506Flannery, T.F.,1994Pages: 240Pages: 240Flannery, T.F.,19952nd Edn.,Pages: 568Pages: 568Flynn, J.J. and M.A. Nedbal,19989414426George, G.G.,1982Phalanger spp.: Their Management in Captivity.]]>1982pp: 67-72pp: 67-72George, G.G.,19871987pp: 507-526pp: 507-526Gilmore, D.P.,2002214193224Glover, I.,1986Pages: 241Pages: 241Groves, C.P.,1987Phalanger Orientalis Group from Indonesian Territory.]]>1987pp: 559-567pp: 559-567Hall, R.,19981998pp: 99-131pp: 99-131Hamilton, A.T. and M.S. Springer,1999Phalanger gymnotis, in the tribe Phalangerini (Marsupialia: Phalangeridae).]]>6117Helgen, K.M.,2003Melomys rufescens paveli.]]>261165172Helgen, K.M. and T.F. Flannery,2004Spilocuscus, with description of a new species from Papua.]]>85825833Isaac, J.L. and C.N. Johnson,2003Trichosurus vulpecula.]]>14818822Jones, A.M.,1997Pages: 197Pages: 197Karr, J.R.,19904391397Kirsch, J.A., M.S. Springer and F.J. Lapointe,199745211280Kocher, T.D., W.K. Thomas, A. Meyer, S.V. Edwards, S. Paabo, F.X. Villablanca and A.C. Wilson,19898661966200Latinis, K.,199671732Laurance, W.F.,199157989McKay G.M. and J.W. Winter,19891989Menzies, J.I.,1991Pages: 138Pages: 138Murphy, W.J., E. Eizirik, S.J. O'Brien, O. Madsen and M. Scally et al.,200129423482351Norris, C.A.,19941117398Norris, C.A.,1999Phalanger lullulae.]]>62014Osborne, M.J. and L. Christidis,200250135149Petocz, R.G.,19941994Primack, R.B., J. Supriatna, M. Indrawan and P. Kramadibrata,19981998Raterman, D., R.W. Meredith, L.A. Ruedas and M.S. Springer,2006BRCA1.]]>54353361Ruedas, L.A. and J.C. Morales,200586353365Springer, M.S., J.A.W. Kirsch, K. Aplin and T. Flannery,199030298311Starr, C.,1990Stilling, P.,19962nd Edn.,Tate, G.H.H.,1945Pseudocheirus and its subgenera.]]>1287130Thompson, J.D., D.G. Higgins and T.J. Gibson,19942246734680Orekhov, V., A. Poltoraus, L.A. Zhivotovsky, V. Spitsyn, P. Ivanov and N. Yankovsky,1999445197201Widayanti, R., H. Wijayanto, W.D. Wendo and R.M. Kunda,201516227235, (In Indonesian)Kumar, S., K. Tamura, I.B. Jakobsen and M. Nei,20011712441245Kirsch, J.A.W. and M.A. Wolman, 2001Ailurops ursinus (Marsupialia: Phalangeridae).]]>232330