Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2016.250.258Serratia plymuthica as Potential Candidate for an Antianthracnose]]>AisyahSiti Nur HarnasHafid SulastriSulastri RetmiRetmi FuaddiHelmi FatchiyahFatchiyah BakhtiarAmri JamsariJamsari 62016196Background and Objective: A new rhizobacteria isolate of Serratia plymuthica (strain UBCR_12) exhibited a promising potential as a biocontrol agent for anthracnose causing agent Colletotrichum gloeosporioides. The aim of this study was to characterize its antagonistic activity and explore the factors contributing to a higher inhibition activity. Materials and Methods: The antifungal effect of UBCR_12 against C. gloeosporioides was assayed under various pH values and nutritional sources. Culture supernatant obtained from UBCR_12 and C. gloeosporioides co-culture was also tested for its inhibitory activity. In addition, the antagonistic range of this isolate was examined against Sclerotium rolfsii and Fusarium oxysporum. Statistical analysis was done using one way analysis of variance and further processed using Fishers Least Significant Difference (LSD) test with a p<0.05. Results: The UBCR_12 induced inhibition was shown to be stable over time at pH 7, while peptone addition led to a faster induction (2 days after treatment) and glucose treatment to a higher activity. Of all these modifications, preliminary co-culture experiments with fungal cells resulted in the best antagonistic activity of UBCR_12 culture supernatant of about 30.66%. This isolate also showed a wide range of antagonistic activity due to its high suppression against S. rolfsii and F. oxysporum from soybean. Conclusion: Both environmental and biotic manipulations contributed an elevated inhibition rate of UBCR_12 against C. gloeosporioides. A proportional combination of the factors stimulating antagonistic activity of this strain is recommended to be utilized for the development of this strain as an antianthracnose. The enhanced antifungal effects of UBCR_12 resulted under each type of modification were varied indicating the difference of cell responses. It suggests that certain antifungal mechanism could be generated by modifying the environmental factor required for its induction. In addition, the application of cell-free culture supernatant provides an alternative solution in the utilization of biocontrol agents. For large scale application, it could minimize the risk of population outbreaks and harmful effects due to the living cells application.]]>Shen, S.S., O.H. Choi, S.H. Park, C.G. Kim and C.S. Park,2005216467Kamensky, M., M. Ovadis, I. Chet and L. Chernin,2003Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases.]]>35323331Li, P., A.H. Kwok, J. Jiang, T. Ran, D. Xu, W. Wang and F.C. Leung,2015Serratia marcescens FS14 reveals its high antagonistic potential.]]>2015Czajkowski, R., W.J. de Boer, J.A. van Veen and J.M. van der Wolf,2012Serratia plymuthica A30 and blackleg-causing Dickeya sp. (biovar 3) in potato (Solanum tuberosum).]]>61677688Gkarmiri, K., R.D. Finlay, S. Alstrom, E. Thomas, M.A. Cubeta and N. Hogberg,2015Rhizoctonia solani AG-3 in response to the antagonistic bacteria Serratia proteamaculans and Serratia plymuthica.]]>2015Chernin, L., A. Brandis, Z. Ismailov and I. Chet,1996Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens.]]>32208212Demain, A.L.,19981259264Pang, Y., X. Liu, Y. Ma, L. Chernin, G. Berg and K. Gao,2009Serratia plymuthica are dependent on N-acyl homoserine lactones.]]>124261268Thomson, N.R., M.A. Crow, S.J. McGowan, A. Cox and G.P.C. Salmond,2000Serratia is under quorum sensing control.]]>36539556Liu, X., J. Jia, R. Popat, C.A. Ortori and J. Li et al.,2011Serratia plymuthicastrain G3: Differential control of motility and biofilm formation according to life-style.]]>2011Freeman, S., T. Katan and E. Shabi,1998Colletotrichum species responsible for anthracnose diseases of various fruits.]]>82596605Prusky, D.,199634413434Syafriani, E., F. Riwany, R. Kamelia, I. Ferita, F. Fatchiyah and J. Jamsari,2016Colletotrichum gloeosporioides.]]>722022209Islam, M.R., Y.T. Jeong, Y.S. Lee and C.H. Song,2012Bacillus subtilis C9 inhibiting the growth of plant pathogenic fungi.]]>405966Zhang, C., X. Zhang and S. Shen,2014Bacillus subtilis KB-1122 on Magnaporthe grisea P131.]]>3017631774Jangpromma, N., S. Kitthaisong, S. Daduang, P. Jaisil and S. Thammasirirak,200774454Nouwens, A.S., M.D.P. Willcox, B.J. Walsh and S.J. Cordwell,2002Pseudomonas aeruginosa.]]>213251346Duffy, B.K. and G. Defago,1999Pseudomonas fluorescens biocontrol strains.]]>6524292438Raaijmakers, J.M., M. Vlami and J.T. de Souza,200281537547El-Banna, N.M.,2006Corynebacterium kutscheri and Corynebacterium xerosis.]]>5833835Nguyen, M.T. and S.L. Ranamukhaarachchi,2010Ralstonia solanacearum in tomato and pepper.]]>92395405Frankowski, J., M. Lorito, F. Scala, R. Schmid, G. Berg and H. Bahl,2001Serratia plymuthica HRO-C48.]]>176421426Wang, S.L., J.H. Peng, T.W. Liang and K.C. Liu,2008Serratia marcescens TKU011.]]>34313161323Babashpour, S., S. Aminzadeh, N. Farrokhi, A. Karkhane and K. Haghbeen,2012Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens.]]>50722735Okay, S., M. Ozdal and E.B. Kurbanoglu,2013Serratia marcescens MO-1]]>37639644Sanchez, S., A. Chavez, A. Forero, Y. Garcia-Huante and A. Romero et al.,201063442459Bibb, M.J.,20058208215Sole, M., A. Francia, N. Rius and J.G. Loren,1997Serratia marcescens.]]>258184Martin, J.F. and A.L. Demain,198044230251Chakrabortty, S., S. Bhattacharya and A. Das,2012Serratia marcescens.]]>2820Cang, S., M. Sanada, O. Johdo, S. Ohta, Y. Nagamatsu and A. Yoshimoto,2000Serratia marcescens grown on ethanol.]]>2217611765Tawiah, A.A., S.Y. Gbedema, F. Adu, V.E. Boamah and K. Annan,20122012Wang, K., P.S. Yan and L.X. Cao,2014Serratia marcescens JPP1 for biocontrol of aflatoxin: Molecular characterization and production optimization using response surface methodology.]]>2014Kurbanoglu, E.B., M. Ozdal, O.G. Ozdal and O.F. Algur,2015Serratia marcescens MO-1 using ram horn peptone.]]>46631637Merrick, M.J. and R.A. Edwards,199559604622Gesheva, V., V. Ivanova and R. Gesheva,2005Streptomyces hygroscopicus.]]>160243248Shapiro, S.,19891989pp: 135-211pp: 135-211Zarei, M., S. Aminzadeh, H. Zolgharnein, A. Safahieh and M. Daliri et al.,2011Serratia marcescens B4A.]]>4210171029Xia, J.L., J. Xiong, R.Y. Zhang, K.K. Liu, B. Huang and Z.Y. Nie,2011Serratia marcescens XJ-01.]]>51301306Benhamou, N. and I. Chet,1997Trichoderma harzianum and Pythium ultimum.]]>6320952099Boonchan, S., M.L. Britz and G.A. Stanley,20006610071019Strom, K., J. Schnurer and P. Melin,2005Lactobacillus plantarum MiLAB 393 and Aspergillus nidulans, evaluation of effects on fungal growth and protein expression.]]>246119124Vinale, F., E.L. Ghisalberti, K. Sivasithamparam, R. Marra and A. Ritieni et al.,2009Trichoderma harzianum secondary metabolites during the interaction with different plant pathogens.]]>48705711Berg, G.,2000Serratia plymuthica strains.]]>88952960Levenfors, J.J., R. Hedman, C. Thaning, B. Gerhardson and C.J. Welch,2004Serratia plymuthica A 153.]]>36677685Dandurishvili, N., N. Toklikishvili, M. Ovadis, P. Eliashvili and N. Giorgobiani et al.,2011Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants.]]>110341352Okamoto, H., Z. Sato, M. Sato, Y. Koiso, S. Iwasaki and M. Isaka,1998Serratia marcescens F-1-1, a biocontrol agent of damping-off of cucumber and antimicrobial activity against other plant pathogens.]]>64294298Shoji, J.I., H. Hinoo, R. Sakazaki, T. Kato and T. Hattori et al.,1989Serratia plymuthica.]]>42869874