Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2016.299.305Cinnamomum kanehirae Cuttings Associated with its Dark Septate Endophytes]]>LinLei-Chen 72016197Background and Objective: Stout camphor tree (Cinnamomum kanehirae Hay.) is an endemic specie in Taiwan and cutting is the major propagation of C. kanehirae for plantation. Mycorrhiza can accelerate the growth of the host plant, especially in root of the host plant. The objective of this study was to investigate the growth effect of the 2 dark septate endophytes isolated from C. kanehirae. Materials and Methods: To measure the effects of stains CkDB2 and CkDB5 on growth performance of cuttings, the cuttings were carefully removed from their substrate after 9 months of incubation. Each treatment had three replicates. Results: After 9 month incubation, the mycorrhizal synthesis experiment showed that the roots of synthesized cuttings produced microsclerotia, a characteristic of dark septate endophyte, but nothing was found in the control. All inoculated cuttings had higher values of net height growth, dry weight, leaf area and chlorophyll concentration than the control. Conclusion: This study demonstrated that the 2 endophytes, strains CkDB2 and CkDB5, capable of forming microsclerotia with C. kanehirae cuttings were dark septate endophytes. Based on the results, CkDB5 had a better growth response than CkDB2. Cuttings inoculated with CkDB5 showed a 200% increase in the root dry weight and therefore, CkDB5 could presumably be a prerequisite for the survival of C. kanehirae cutting plantation.]]>Liao, J.C.,1996Cinnamomum.]]>1996pp: 437-448pp: 437-448Chang, T.T. and W.R. Wang,2005Antrodia cinnamomea on artificial agar media.]]>46151154Chang, S.H., C.K. Ho and J.Y. Tsay,2002In vitro culture of Cinnamomum kanehirae Hay.]]>17491501, (In Chinese)Lin, L.C., T.P. Chang, S.L. Hong, C.K. Hsieh, J.L. Chen and T.Y. Tseng,2015Cinnamomum kanehirae cuttings and its root fungal endophytes.]]>48127136, (In Chinese)Kao, Y.P. and S.G. Huang,1993Cinnamomum kanehirae hag.]]>8371388, (In Chinese)Yang, J.C.,2013Cinnamomum kanehirae.]]>203032, (In Chinese)Cairney, J.W.G. and A.A. Meharg,200354735740Schmid, E., F. Oberwinkler and L.D. Gomez,199573991996Azcon-Aguilar, C. and J.M. Barea,199768124Gibson, B.R. and D.T. Mitchell,2005in vitro.]]>15231234Lin, L.C., M.J. Lee and J.L. Chen,2011Rhododendron formosanum Hemsl.).]]>21331339Douds, Jr.D.D., G. Becard, P.E. Pfeffer, L.W. Doner, T.J. Dymant and W.M. Kayser,1995Sciadopitys verticillata Sieb & Zucc. cuttings.]]>30133134Scagel, C.F.,2004129624634Scagel, C.F.,200414355363Scagel, C.F.,2005Leucothoe fontanesiana cuttings to inoculation with ericoid mycorrhizal fungi.]]>80254262Scagel, C.F.,200540786794Oseni, T.O., N.S. Shongwe and M.T. Masarirambi,201012789792Chang, T.T. and W.R. Wang,2008Antrodia cinnamomea.]]>23105110, (In Chinese)Liao, P.C., D.C. Kuo, C.C. Lin, K.C. Ho, T.P. Lin and S.Y. Hwang,2010Cinnamomum kanehirae Hay.(Lauraceae) in Taiwan inferred from nuclear genes.]]>2010Hong, S.L., T.C. Lin and L.C. Lin,2014Cinnamomum kanehirai at Dabang area.]]>47393398, (In Chinese)Chang, C.Y.,2005Cinnamomum kanehirae cuttings.]]>2005Cho, H.Y., C.Y. Chang, L.C. Huang, J.B. Tsai and Z.H. Liu,2011Cinnamomum kanehirae.]]>52153160Chen, Y.C.,2013Cinnamomum kanehirae Hayata.]]>2013Lee, C.F.,2013Cinnamomum kanehirae cuttings.]]>2013Varma, A., S. Verma, Sudha, N. Sahay, B. Butehorn and P. Franken,1999Piriformospora indica, a cultivable plant-growth-promoting root endophyte.]]>6527412744Zhang, H.H., M. Tang, H. Chen and Y.J. Wang,2012Lycium barbarum L.]]>509196McGonigle, T.P., M.H. Miller, D.G. Evans, G.L. Fairchild and J.A. Swan,1990115495501Plenchette, C., J.A. Fortin and V. Furlan,198370199209Porra, R.J., 2002a and b]]>73149156Barrow, J. and R. Aaltonen,2001Atriplex canescens (Pursh) Nutt. roots by dark septate fungi and the influence of host physiological activity.]]>11199205Barrow, J.R.,2003Bouteloua in arid southwestern USA rangelands.]]>13239247Peterson, R.L., H.B. Massicotte and L.H. Melville,2004Pages: 173Pages: 173Mathew, A. and R.M. Malathy,2008Chlorophytum borivillianum. ]]>679196Berta, G., A. Fusconi and A. Trotta,199333159173Berta, G., A. Trott, A. Fusconi, J.E. Hooker and M. Munro et al.,1995Prunus cerasifera.]]>5281293Verkade, S.D. and D.F. Hamilton,1987Viburnum dentatum L. cuttings.]]>58081Gross, E., L.I.T. Casagrande and F.H. Caetano,2004Pinus caribaea Morelet. var. hondurensis Barr. and Golf. seedlings.]]>1817Herrmann, S., R. Oelmuller and F. Buscot,2004Piloderma croceum: Influence on plant development and photosynthesis.]]>161509517Simard, S.W. and D.M. Durall,20048211401165Sage, R.F., M. Li and R.K. Monson,19994 Photosynthesis.]]>1999pp: 551- 584pp: 551- 584Marks, S. and K. Clay,1996Festuca arundinacea to fungal endophyte infection.]]>133727733Wu, L.Q., Y.L. Lv, Z.X. Meng, J. Chen and S.X. Guo,2010Saussurea involucrata Kar. et Kir.]]>20127135