Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.189.196Yanwirasti A. HarahapWirsma ArisantyDessy 42017204Background: Abnormal expression of several microRNAs (miRNAs) has been demonstrated in many types of cancer tumor tissue. The miR-10b and miR-21 are an oncogenic miRNAs which play role in proliferation and invasion of Breast Cancer (BC) tumorigenesis. The aim of this study was to evaluate the miR-21 and miR-10b expression in BC in West Sumatran women, Indonesia. Materials and Methods: A total of 40 samples, consisting of 30 samples of breast cancer tissues (BC) and 10 samples of fibroadenoma tissues (FATs) as control and non-cancerous were analyzed. The miR-21 and miR-10b expression of each sample were investigated by using realtime PCR, followed by universal Reverse Transcription (RT) then real-time PCR amplification with specific primers. Hsa-miR-16-5p LNA PCR primer was used as an endogenous control. Results: The results showed that the expression level of miR-21 was more than 4 times higher in BC than in FATs. The expression level of miR-10b was lower in BC than FATs, by a factor of 3.34 fold. Both these differences were statistically different (p = 0.001). Conclusion: In this study it was concluded that for this sample of West Sumatran Women miR-21 expression in BC was higher than in FATs, whereas miR-10b was lower in BC than in FATs.]]>Bhati, H.,20162016Bhoo-Pathy, N., C.H. Yip, M. Hartman, C.S.P.M. Uiterwaal and B.C.R. Devi et al.,201349703709Couch, F.J., K.L. Nathanson and K. Offit,2014BRCA: Setting paradigms in personalized cancer care and prevention.]]>34314661470Terry, M.B., J.A. McDonald, H.C. Wu, S. Eng and R.M. Santella,20162016pp: 33-68pp: 33-68Verma, M., S. Rogers, R.L. Divi, S.D. Schully and S. Nelson et al.,201423223233Ahmad, J., S.E. Hasnain, M.A. Siddiqui, M. Ahamed, J. Musarrat and A.A. Al-Khedhairy,2013137680694Lin, S. and R.I. Gregory,201515321333Slack, F.J. and J.B. Weidhaas,200835927202722Paladini, L., L. Fabris, G. Bottai, C. Raschioni, G.A. Calin and L. Santarpia,20162016Meister, G., M. Landthaler, Y. Dorsett and T. Tuschl,200410544550Bajan, S. and G. Hutvagner,201431017Shenouda, S.K. and S.K. Alahari,20092009Wang, W. and Y.P. Luo,2015161831Fu, S.W., L. Chen and Y.G. Man,20112116122George, G.P. and R.D. Mittal,201025414Iorio, M.V. and C.M. Croce,201218215222Lu, Y., J. Yao, J. Yu, Q. Wei and X. Cao,20142014Xiao, H., H. Li, G. Yu, W. Xiao and J. Hu et al.,20143118321838Schickel, R., B. Boyerinas, S.M. Park and M.E. Peter,20082759595974Zhang, Z.J. and S.L. Ma,201227903910Hunt, K.K., L.A. Newman, E.M. Copeland III and K.I. Bland,20102010Harahap, W.A., D. Arisanty, D. Khambri, Yanwirasti and S. Mubarika,201542186190, (In Indonesian)Saito, T. and P. Sætrom,201027243249Fujita, S., T. Ito, T. Mizutani, S. Minoguchi, N. Yamamichi, K. Sakurai and H. Iba,2008378492504Lu, J., G. Getz, E.A. Miska, E. Alvarez-Saavedra and J. Lamb et al.,2005435834838Si, M.L., S. Zhu, H. Wu, Z. Lu, F. Wu and Y.Y. Mo,20072627992803Huang, G.L., X.H. Zhang, G.L. Guo, K.T. Huang and K.Y. Yang et al.,200921673679Medina, P.P., M. Nolde and F.J. Slack,2010in vivo model of microRNA-21-induced pre-B-cell lymphoma.]]>4678690Wickramasinghe, N.S., T.T. Manavalan, S.M. Dougherty, K.A. Riggs, Y. Li and C.M. Klinge,20093725842595Dong, J., Y.P. Zhao, L. Zhou, T.P. Zhang and G. Chen,201142814Shi, L., J. Chen, J. Yang, T. Pan, S. Zhang and Z. Wang,20101352255264Buscaglia, L.E.B. and Y. Li,201130371380Ozgun, A., B. Karagoz, O. Bilgi, T. Tuncel, H. Baloglu and E.G. Kandemir,201336115118Kuang, Y. and Y.J. Nie,20169470473Samatov, T.R., A.G. Tonevitsky and U. Schumacher,20132013Ma, L., J. Teruya-Feldstein and R.A. Weinberg,2007449682688Ahmad, A., S. Sethi, W. Chen, R. Ali-Fehmi, S. Mittal and F.H. Sarkar,20146384390Liao, C.G., L.M. Kong, P. Zhou, X.L. Yang, J.G. Huang, H.L. Zhang and N. Lu,20142014Ma, L., F. Reinhardt, E. Pan, J. Soutschek and B. Bhat et al.,201028341347Gee, H.E., C. Camps, F.M. Buff, S. Colella and H. Sheldon et al.,2008455E8E9Heerboth, S., G. Housman, M. Leary, M. Longacre and S. Byler et al.,20152015