Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.244.252Rumiyati Sismindari SemiartiEndang MilasariAsri Fajar SariDheatika Karina FitrianaNia GaluhSekar 52017205Background and Objective: Dragon fruit (Hylocereus spp.), apple (Malus sylvestris Mill.) and tomato (Solanum lycopersicum L.) are high potential sources of antioxidant compounds such as phenolics. The compounds have the capability of protecting cells and tissues against free radicals. Secondary metabolite produced by callus cell culture from plant organs also acts as a source of antioxidants. This study aimed to determine the optimal ratio of sucrose and 2,4-D in Murashige and Skoog (MS) medium for callus induction from different plant organ explants. With all of characteristic, callus can be used further for the development of natural cell regeneration agent. Methodology: This study was conducted using analytical technique. Suitable explants were obtained. They were developed in various concentrations of combination between MS medium and 2,4-D. Callus growth, including their weight and surface was then measured and analyzed by using one-way analysis of variance (ANOVA). Results: Callus was able to grow from its explants in 5-7 days after induction process. They were clear in color and had friable texture. The highest value of fresh weight of dragon fruit callus was obtained through MS supplemented with 1 μL L1 2,4-D and 30 g sucrose. However, apple and tomato callus induction and growth maintenance reached optimal medium on MS supplemented with 30 g sucrose and 2 μL L1 2,4-D. Conclusion: Callus of apple, dragon fruit and tomato was maintained upon MS supplemented with 30-40 g sucrose and 1-2 μL L1 2,4-D for optimum induction and growth. The optimization of growth medium will give advantages for further development of natural cell regeneration agent.]]>Pandey, K.B. and S.I. Rizvi,20092270278Hariprasath, L., R. Jegadeesh, P. Arjun and N. Raaman,2015In vitro propagation of Senecio candicans DC and comparative antioxidant properties of aqueous extracts of the in vivo plant and in vitro-derived callus.]]>98134141Ortiz-Hernandez, Y.D. and J.A. Carrillo-Salazar,2012Hylocereus spp.): A short review.]]>3220237Rebecca, O.P.S., A.N. Boyce and S. Chandran,2010Hylocereus polyrhizus).]]>914501454Bakar, J., C.E. Shu, S. Muhammad, S. Kharidah and D.M. Hashim,2011Hylocereus polyrhizus) peel.]]>18279285Luo, H., Y. Cai, Z. Peng, T. Liu and S. Yang,2014in vitro evaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of pitaya (dragon fruit) peel.]]>2014Stojiljkovic, D., I. Arsic and V. Tadic,2016Malus sylvestris (L.) Mill., Rosaceae) as a source of antioxidant substances for use in production of nutraceuticals and cosmeceuticals.]]>80165176Chandra, I., P. Singh, A. Bhattacharya, P. Singh, S. Javed and A. Singhamahapatra,2013In vitro callus induction, regeneration and micropropagation of Solanum lycopersicum.]]>2192197Ikeuchi, M., K. Sugimoto and A. Iwase,20132531593173Barbulova, A., F. Apone and G. Colucci,2014194104Osman, N.I., N.J. Sidik and A. Awal,2016Barringtonia racemosa L.]]>6143147Chen, L., A. Bhagsari and J. Carter,2006in vitro morphogenesis of sweet potato.]]>50114117Jones, A.M.P. and P.K. Saxena,2013Artemisia annua L.: A novel approach to reduce oxidative browning in plant tissue culture.]]>2013Guan, C.M., S.S. Zhu, X.G. Li and X.S. Zhang,2006Arabidopsis callus in vitro.]]>2511331137Vaux, D.L.,2012492180181Lee, S.T. and W.L. Huang,2014Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation.]]>73193204Mazumdar, P., A. Basu, A. Paul, C. Mahanta and L. Sahoo,2010Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L.]]>76337344Chaffey, N.,201010536Yaacob, J.S., N. Mahmad, R.M. Taha, N. Mohamed, A.I.M. Yussof and A. Saleh,20142014Indah, P.N. and D. Ermavitalini,2013Calophyllum inophyllum Linn.) pada beberapa kombinasi Konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic acid (2,4-D).]]>2E1E6Tahir, S.M., K. Victor and S. Abdulkadir,2011Saccharum officinarum).]]>19213217Delporte, F., A. Pretova, P. Du Jardin and B. Watillon,201425114551470