Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.365.371Panulirus polyphagus (Herbst, 1793)]]>Nor FatihahSiti Muhd-FaroukHarman Amin-SafwanAdnan Hafiz MahsolHairul IkhwanuddinMhd 72017207Background and Objective: Mud spiny lobsters, Panulirus polyphagus (P. polyphagus) are one of the most important fisheries resources now-a-days due to quality that its possessed. However, there is still lack of in-depth study about this species mainly on males testicular characteristics and sexual maturity size. Therefore, the present study was carried out to investigate the histological characteristics on the testes and sexual maturity sizes of mud spiny lobster, Panulirus polyphagus. Materials and Methods: The testes were dissected out and fixed in 10% buffered formalin solution for 11 h, dehydrated in 70% alcohol and lastly placed in tissue processor for 18±1 h at 60°C. The tissues blocks were cut at the thickness of 4 μm on a rotary microtome. Stained tissues were taken under Advance Microscope (Nikon Eclipse 80i Nomarski DIC). Collected data were analyzed using Microsoft Excel 2013. Data were presented as mean±standard deviation. Statistical analyses were done using one-way ANOVA using SPSS (Version 22). Results: These lobules of mature P. polyphagus were formed via different germinative lineage cells such as spermatogonia, spermatocytes, spermatids and spermatozoa. The histological characteristics of testes showed that the process of spermatogenesis went through the stages of four testes maturation which were spermatogonia I and II, spermatocytes I and II, spermatids and spermatozoa stages within different body weight of P. polyphagus. It was found that there were significant difference between body weight and carapace length to the testicular maturation stages (one-way ANOVA and p = 0.000). Conclusion: The results of this experiment indicated that males P. polyphagus have four stages of testes maturation and can be considered to have fully mature testes that ready for fertilization at 452 g body weight (BW) and 107 mm carapace length (CL) or more.]]>Phillips, B.F.A., J.S. Cobb and R.W. George,19801980pp: 1-82pp: 1-82Ikhwanuddin, M., S.N. Fatihah, J.R. Nurul, M.Z. Zakaria and A.B. Abol-Munafi,2014Panulirus polyphagus (Herbst, 1793) from Johor coastal water of Malaysia.]]>3120792086Tuan, L.A. and N.D. Mao,20042004pp: 2430Wahyudin, R.A., Y. Wardiatno, M. Boer, A. Farajallah and A.A. Hakim,2017Panulirus polyphagus (Herbst, 1793)(Crustacea, Achelata, Palinuridae) in Mayalibit bay, West Papua, Indonesia.]]>18780783Fatihah, S.N., S. Jasmani, A.B. Abol-Munafi, S. Noorbaiduri, H. Muhd-Farouk and M. Ikhwanuddin,2016Panulirus polyphagus.]]>4625663Fatihah, S.N., J. Safiah, A.B. Abol-Munafi and M. Ikhwanuddin,2015Panulirus polyphagus.]]>103544Fatihah, S.N., J. Safiah, A.B. Abol-Munafi and M. Ikhwanuddin,2014Panulirus polyphagus).]]>1711241129Fatihah, S.N., J. Safiah and A.B. Abol-Munafi and M. Ikhwanuddin,2014Panulirus polyphagus.]]>17937941Ikhwanuddin, M., S.N. Fatihah, A.H. Nurfaseha, R. Ruhinisa and Z.Z. Tira et al.,2015Panulirus polyphagus.]]>10244254Ikhwanuddin, M., S.N. Fatihah, A.H. Nurfaseha, M. Fathiah and M. Effenddy et al.,2014Panulirus polyphagus.]]>9113Krol, R.M., W.E. Hawkins and R.M. Overstreet,1992Vol. 10,pp: 295-343pp: 295-343Castilho, G.G., A. Ostrensky, M.R. Pie and W.A. Boeger,2008Ucides cordatus (L.) (Crustacea, Brachyura, Ocypodidae).]]>161157161Garcia, T.M. and J.R.F. Silva,2006Goniopsis cruentata) (Latreille, 1803).]]>49339345De Lima, A.V.P. and T.C.V. Gesteira,2008Panulirus laevicauda (Latreille, 1817).]]>51701709Lawton, P. and K.L. Lavalli,1995Homarus americanus: Postlarval, Juvenile, Adolescent and Adult Ecology.]]>pp: 47-88pp: 47-88Lipcius, R.N., M.L. Edwards, W.F. Herrnkind and S.A. Waterman,1983In situ mating behavior of the spiny lobster Panulirus argus.]]>3217222George, R.W. and G.R. Morgan,1979Panulirus versicolor) as a method for determining size at first physical maturity.]]>175182185Muhd-Farouk, H., S. Jasmani and M. Ikhwanuddin,2016Scylla olivacea (Herbst, 1796).]]>4517886IBM.,2014Sherkhane, U.D., M.U. Patil and G.S. Pande,2010Barytelphusa cunicularis (Westwood 1836) (Deacapoda: Crustacea).]]>5599603Amin-Safwan, A., H. Muhd-Farouk, M. Nadirah and M. Ikhwanuddin,2016Scylla olivacea (Herbst, 1796) in captivity.]]>19219226