Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.436.441Bacillus subtilis FNCC 0059 Oxalate Decarboxylase Activity]]>Nur Indah KoniTheresia Rusman HanimChusnul Zuprizal 92017209Background and Objective:Bacillus subtilis is a bacterium that can produce the oxalate decarboxylase (ODC) enzyme. This enzyme decomposes oxalate. ODC enzyme activity is influenced by temperature and pH. This study was conducted to determine the optimum pH and temperature for ODC enzyme activity in B. subtilis FNCC 0059. Materials and Methods:B. subtilis was cultivated in oxalate medium at different incubation temperatures for 24 h. The tested temperatures were 25, 29, 33, 37, 41 and 45°C. In separate experiments, cultures were grown in oxalate medium at varying pH from 4-8.5. The cell growth assay and enzyme activity were performed and all the experiments were done with 3 replicates. Data were analyzed using one-way analysis of variance. Results: The optimum temperature of 37°C could produce maximum ODC enzyme activity (0.041 U mL1) enzyme activity increased to the point of the optimum pH and then decreased 5.6% when the pH was increased 0.5 from the optimum. Maximum ODC enzyme activity (0.0413 U mL1) was achieved at pH 5.5. Conclusion: Temperature and the pH of the medium affected ODC enzyme activity. Optimum medium condition of B. subtilis was reached at 37°C and pH 5.5.]]>Cheeke, P.R. and L.R. Shull,1985pp: 313-320pp: 313-320Svedruzic, D., S. Jonsson, C.G. Toyota, L.A. Reinhardt, S. Ricagno, Y. Lindqvist and N.G. Richards,2005433176192Rahman, M.M., R.B. Abdullah and W.E.W. Khadijah,201397605614Paul, V., S. Verma, Sushma and A. Paul,20125311314Giardina, S., C. Scilironi, A. Michelotti, A. Samuele, F. Borella, M. Daglia and F. Marzatico,2014In vitro anti-Inflammatory activity of selected oxalate-degrading probiotic bacteria: Potential applications in the prevention and treatment of hyperoxaluria.]]>79384390Sasikumar, P., S. Gomathi, K. Anbazhagan, A. Abhishek and E. Paul et al.,2014Lactobacillus plantarum expressing and secreting heterologous oxalate decarboxylase prevents renal calcium oxalate stone deposition in experimental rats.]]>2014Campieri, C., M. Campieri, V. Bertuzzi, E. Swennen and D. Matteuzzi et al.,20016010971105Gomathi, S., P. Sasikumar, K. Anbazhagan, S. Sasikumar, M. Kavitha, M.S. Selvi and G.S. Selvam,20142014Anbazhagan, K., C.E. Raja and G.S. Selvam,2007Paracoccus alcaliphilus isolated from Amorphophallus sp. rhizoplane.]]>2315291535Turroni, S., B. Vitali, C. Bendazzoli, M. Candela and R. Gotti et al.,2007Lactobacillus acidophilus.]]>10316001609Burrell, M.R., V.J. Just, L. Bowater, S.A. Fairhurst, L. Requena, D.M. Lawson and S. Bornemann,2007461232712336Lee, E., B.C. Jeong, Y.H. Park and H.H. Kim,2014Bacillus subtilis and characterization of the recombinant enzyme.]]>2014Molina, L., T. Goodall, U. Twahir, E. Moomaw and A. Angerhofer,2015Bacillus subtilis oxalate decarboxylase and Ceriporiopsis subvermispora oxalate oxidase using a luminescent oxygen sensor.]]>5826831Makela, M., S. Galkin, A. Hatakka and T. Lundell,200230542549Prescott, L.M., J.P. Harley and D.A. Klein,19994th Edn.,pp: 55-90pp: 55-90Waleed, M., A.A. Faiza and I. Nizar,2015Bacillus cereus isolated from Syrian sources.]]>2218241830Jiang, Z.L., M.X. Zhao and L.X. Liao,1996320139143Jin, Z.X., C. Wang, W. Chen, X. Chen and X. Li,2007Pandoraea sp. OXJ-11 and its ability to protect against Sclerotinia sclerotiorum infection.]]>5313161322Tanner, A. and S. Bornemann,2000Bacillus subtilis YvrK is an acid-induced oxalate decarboxylase.]]>18252715273Gasperz, V.,19912nd Edn.,pp: 123-130pp: 123-130Mahazar, N.H., Z. Zakuan, Y. Rukayadi, H. Norhayati and A. Meorhussin,2017Candida sp. and Blastobotrys sp. as starter culture in fermentation of cocoa beans (Theobroma cacao) using Response Surface Methodology (RSM).]]>20154159Hogg, S.,2005pp: 90-105pp: 90-105Kusumadjaja, A.P. and R.P. Dewi,2010Carica papaya).]]>5147151Van Eunen, K. and B.M. Bakker,2014in vivo-like enzyme kinetics.]]>1126130Bisswanger, H.,201414155Anand, R., P.C. Dorrestein, C. Kinsland, T.P. Begley and S.E. Ealick,2002Bacillus subtilis at 1.75 A resolution.]]>4176597669Dutton, M.V., M. Kathiara, I.M. Gallagher and C.S. Evans,1994Coriolus versicolor.]]>116321325Graz, M., K. Rachwal, R. Zan and A. Jarosz-Wilkolazka,2016Abortiporus biennis.]]>63595600Eed, J.,2013104750Zhu, C.X. and F. Hong,2010Trametes versicolor by addition of inorganic acids.]]>160655664Anbazhagan, K., P. Sasikumar, S. Gomathi, H.P. Priya and G.S. Selvam,2013In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase.]]>115880887Daniel, R.M., M.E. Peterson, M.J. Danson, N.C. Price and S.M. Kelly et al.,2010425353360