Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.52.58Halomonas spp., the Potential Nitrifying Bacteria for Saline Ammonium Waste Water Treatment]]>SangnoiYutthapong ChankaewSunipa O-ThongSompong 12017201Background and Objective: Toxic nitrogen compounds are one cause decreasing of shrimp production and water pollution. Indigenous Halomonas spp., isolated from Pacific white shrimp farm are benefitted for saline ammonium waste water treatment. This study aimed to isolate the heterotrophic-halophilic Halomonas spp. and investigate their ammonium removal efficiency. Materials and Methods:Halomonas spp., were isolated by culturing of samples collected from shrimp farm into modified Pep-Beef-AOM medium. Ammonium converting ability was tested and monitored by nitrite reagent. Ammonium removal efficiency was measured by the standard colorimetric method. Identification and classification of Halomonas spp., were studied by morphological, physiological and biochemical characteristics as well as molecular information. Results: There were 5 strains of heterotrophic-halophilic nitrifying bacteria including SKNB2, SKNB4, SKNB17, SKNB20 and SKNB22 were isolated. The identification result based on 16S rRNA sequence analysis indicated that all 5 strains were Halomonas spp., with sequence similarity values of 91-99 %. Ammonium removal efficiency of all strains showed a range of 23-71%. The production of nitrite was low detected of 0.01-0.15 mg-N L1, while the amount of nitrate was almost undetectable. Conclusion: This might suggest that the indigenous Halomonas spp., as nitrifying bacteria involved biological nitrification process for decreasing and transforming of ammonia. Due to being heterotrophic, halophilic and ammonium removing bacteria, these Halomonas spp., could be developed for use in treatment of saline ammonium waste water.]]>Achuthan, C., V.R. Kumar, N.J. Manju, R. Philip and I.B.S. Singh,200635240248Ziembinska, A., S. Ciesielski and K. Miksch,200955373380Paungfoo, C., P. Prasertsan, P.C. Burrell, N. Intrasungkha and L.L. Blackall,2007In situ Hybridization (FISH), 16S rRNA gene cloning and phylogenetic analysis.]]>97985990Ahn, Y.H.,20064117091721Kim, D.J., D.I. Lee and J. Keller,200697459468Kim, K.K., L. Jin, H.C. Yang and S.T. Lee,2007Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water.]]>57675681Li, H.B., L.P. Zhang and S.F. Chen,2008Halomonas korlensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from saline and alkaline soil.]]>5825822588Yang, X.P., S.M. Wang, D.W. Zhang and L.X. Zhou,2011Bacillus subtilis A1.]]>102854862Lu, Y., X. Wang, B. Liu, Y. Liu and X. Yang,2012209951002Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar,20133027252729Strickland, J.D.H. and T.R. Parsons,19722nd Edn.,Pages: 310Pages: 310Hedi, A., N. Sadfi, M.L. Fardeau, H. Rebib, J.L. Cayol, B. Ollivier and A. Boudabous,20092009Gonzalez-Domenech, C.M., V. Bejar, F. Martinez-Checa and E. Quesada,2008Halomonas nitroreducens sp. nov., a novel nitrate- and nitrite-reducing species.]]>58872876Guo, Y., X. Zhou, Y. Li, K. Li and C. Wang et al.,2013Halomonas campisalis.]]>3520452049Wen, Y. and C.H. Wei,20111069856990He, T., Z. Li, Q. Sun, Y. Xu and Q. Ye,2016Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion.]]>200493499Sun, Z., Y. Lv, Y. Liu and R. Ren,2016Cupriavidus sp. S1.]]>220142150Purkhold, U., A. Pommerening-Roser, S. Juretschko, M.C. Schmid, H.P. Koops and M. Wagner,2000amoA sequence analysis: Implications for molecular diversity surveys.]]>6653685382Cui, Y.W., H.Y. Zhang, J.R. Ding and Y.Z. Peng,20162016Zhang, L., K. Mai, B. Tan, Q. Ai and C. Qi et al.,2009Halomonas sp. B12 on the intestinal microflora, immunological parameters and midgut histological structure of shrimp, Fenneropenaeus chinensis.]]>405866Suantika, G., P. Aditiawati, D.I. Astuti and Z.F. Khotimah,2013Halomonas aquamarina and Shewanella algae for white shrimp (Litopenaeus vannamei Boone) hatchery productivity in zero water discharge system.]]>2013