Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2018.432.440Sophora japonica Seeds]]>NabiMudasar BhatAaliya Abeer RasoolShayaq ul AshrafSairish MaqboolRouf Ahmad GanieShowkat AminShajrul 92018219Background and Objective: Protease inhibitors (PIs) regulate various cellular processes like cell cycle, differentiation, apoptosis and immune responses. Leguminous seeds are rich sources of protease inhibitors and many novel protease inhibitors have been purified from them. To isolate and purify protease inhibitors from seeds of Sophora japonica, characterize and investigate their anti- microbial activity. Materials and Methods: Protease inhibitors (SJ-pi I and SJ-pi II) were purified to homogeneity by ammonium sulfate precipitation, Ion exchange chromatography and column chromatography. The molecular mass was estimated by size exclusion chromatography and by SDS-PAGE and anti- microbial activity was tested by agar disk diffusion method. Results: Two protease inhibitors were isolated and purified from Sophora japonica seeds, SJ-pi I and SJ-pi II, with molecular weight of 15.1 and 31 kDa, respectively. Both purified inhibitors were active over a range of pH (6.0-9.0) and showed maximum activity in the temperature range of 30-40°C. They inhibited the growth of three Gram-positive bacteria. Conclusion: Protease inhibitors were classified as serine protease inhibitors, however further necessary structural investigations need to be carried out so as to group them into specific class of serine protease inhibitors.]]>Yang, Y., H. Hong, Y. Zhang and W. Cai,20092009Hsieh, C.C., B. Hernandez-Ledesma, H.J. Jeong, J.H. Park and O. Ben,20102010Turk, B.,20065785799Drag, M. and G.S. Salvesen,20109690701Shen, A.,2010614311443Hossain, M.S., A.K. Azad, S.M. Abu Sayem, G. Mostafa and M.M. Hoq,2007Bacillus licheniformis MZK-3.]]>7599606Rakashanda, S., S. Mubashir, Y. Qurishi, A. Hamid, A. Masood and S. Amin,2013Lavatera cashmeriana Camb. seeds: Isolation, characterization and in-vitro cytoxicity activity.]]>25565Gandreddi, V.S., V.R. Kappala, K. Zaveri and K. Patnala,2015Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization.]]>2015Koiwa, H., R.A. Bressan and P.M. Hasegawa,19972379384Cruz, A.C., F.S. Massena, L. Migliolo, L.L. Macedo and N.K. Monteiro et al.,2013Piptadenia moniliformis) seeds.]]>706168Smid, I., A. Rotter, K. Gruden, J. Brzin and M.B. Gasparic et al.,20151225966Sels, J., J. Mathys, B.M.A. de Coninck, B.P.A. Cammue and M.F.C. de Bolle,200846941950Deu, E., M. Verdoes and M. Bogyo,201219916Clemente, A., F.J. Moreno, M.D.C. MarinâManzano, E. Jimenez and C. Domoney,2010Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases.]]>54396405Lin, J.Y., S.C. Chu, H.C. Wu and Y.S. Hsieh,1991Acacia confusa.]]>110879883Birk, Y., A. Gertler and S. Khalef,196387281284Bonev, B., J. Hooper and J. Parisot,20086112951301Guimaraes, L.C., C.F.R. de Oliveira, S. Marangoni, D.G.L. de Oliveira and M.L.R. Macedo,2015Poincianella pyramidalis with insecticide activity against the Mediterranean flour moth.]]>11819Dantzger, M., I.M. Vasconcelos, V. Scorsato, R. Aparicio, S. Marangoni and M.L.R. Macedo,2015Clitoria fairchildiana seeds: Isolation, biochemical properties and insecticidal potential.]]>118224235Habib, H., K. Fazili, M. Zargar and B. Ganie,2016Pisum sativum L. cv. Arkel.]]>4172179Clemente, A., M. del Carmen Marin-Manzano, M. del Carmen Arques and C. Domoney,20132013Migliolo, L., A.S. de Oliveira, E.A. Santos, O.L. Franco and P. Mauricio,2010Kunitz trypsin inhibitor from Adenanthera pavonina L. seeds with double activity toward serine-and cysteine-proteinases.]]>29148156De Paula, C.A.A., V.J. Coulson-Thomas, J.G. Ferreira, P.K. Maza and E. Suzuki et al.,2012Enterolobium contortisiliquum trypsin inhibitor (EcTI), a plant proteinase inhibitor, decreases in vitro cell adhesion and invasion by inhibition of Src protein-Focal Adhesion Kinase (FAK) signaling pathways.]]>287170182Clemente, A., J.M. Gee, I.T. Johnson, D.A. MacKenzie and C. Domoney,2005Pisum sativum L.) protease inhibitors from the bowman-Birk class influence the growth of human colorectal adenocarcinoma HT29 cells in vitro.]]>5389798986Chevreuil, L.R., J.F.D.C. Goncalves, L.D.A. Calderon, L.A.G.D. Souza and S.C. Pando,201441181186Sharma, P., A.K. Nath, R. Kumari, S.V. Bhardwaj,2012Albizia lebbeck seeds.]]>23131137Fook, J.M.S.L.L., L.L.P. Macedo, G.E.D.D. Moura, F.M. Teixeira, A.S. Oliveira, A.F.S. Queiroz and M.P. Sales,2005Tamarindus indica seeds and its effects on the release of human neutrophil elastase.]]>7628812891Sivakumar, S., O.L. Franco, P.D. Tagliari, C. Bloch, Jr., M. Mohan and B. Thayumanavan,2005Prosopis juliflora seeds with activity toward pest digestive enzymes.]]>12561565Zhou, J.Y., H. Liao, N.H. Zhang, L. Tang, Y. Xu and F. Chen,2008Albizzia kalkora and its inhibitory effect against pest midgut proteases.]]>3014951499Kansal, R., R.N. Gupta, K.R. Koundal, K. Kuhar and V.K. Gupta,2008Vigna radiate L. Wilczek) seeds.]]>2008Godbole, S.A., T.G. Krishna and C.R. Bhatia,1994Cajanus cajan (l) millsp) seeds.]]>648793Bhattacharyya, A., S. Rai and C.R. Babu,200745169177Sawano, Y., K.I. Hatano, T. Miyakawa, H. Komagata, Y. Miyauchi, H. Yamazaki and M. Tanokura,200814619091919