Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2020.1086.1095PashaFarhina AlatawiAishah AmirManal FaridiUzma 82020238Background and Objective: The epidemiology of Nipah virus (NiV) was shortly seen in many Asian countries like Malaysia, Bangladesh and India most recently. Nipah virus also synonym as bat born virus is transmitted primarily by fruit bats. The 2 different strains transmitted are Hendra (highly pathogenic) and Cedar (non-pathogenic). The present study was attempt to develop recombinant protein based reagents for molecular diagnosis of Nipah. Materials and Methods: The different primer sets were developed using bioinformatics software DNASTAR. The E. coli cells were used for recombinant protein expression. Results: The NiV ‘G’ region primers were designed and amplified for 1 kb fragment and cloned. The NiV ‘G’ fragments were sub-cloned in pET-28(+) B and pGEX-5x-1. Recombinant protein thus obtained in soluble form in both the cases was essayed using western blot. The result showed the protein expression yield was more in pET-28(+) B with low stability and vice versa for pGEX-5x-1. Conclusion: The antibodies raised from the protein can be used as diagnostic reagent for detection of NiV. Thus, a new diagnostic technique can be industrialized.]]>Wang, L.F., W.P. Michalski, M. Yu, L.I. Pritchard, G. Crameri, B. Shiell and B.T. Eaton,19987214821490Daniels, P., T. Ksiazek and B.T. Eaton,20013289295Parashar, U.D., L.M. Sunn, F. Ong, A.W. Mounts and M.T. Arif et al.,2000Nipah virus, during a 1998-1999 outbreak of severe encephalitis in Malaysia.]]>18117551759Halpin, K., P.L. Young, H.E. Field and J.S. Mackenzie,20008119271932Wacharapluesadee, S., B. Lumlertdacha, K. Boongird, S. Wanghongsa and L. Chanhome et al.,20051119491951Harcourt, B.H., A. Tamin, T.G. Ksiazek, P.E. Rollin, L.J. Anderson, W.J. Bellini and P.A. Rota,2000271334349WHO.,20012001Chadha, M.S., J.A. Comer, L. Lowe, P.A. Rota and P.E. Rollin et al.,200612235240Chua, K.B., C.L. Koh, P.S. Hooi, K.F. Wee and J.H. Khong et al.,20024145151Chua, K.B.,200326265275Lam, S.K. and K.B. Chua,200234S48S51Nor, M.M., C.H. Gan and B.L. Ong,200019160165Chua, K.B.,20035487490Guillaume, V., H. Contamin, P. Loth, M.C. Georges-Courbot and A. Lefeuvre et al.,200478834840Kumar, S.,20032003Sambrook, S., E.F. Fritch and T. Maniatis,19892nd Edn.,Hsu, V.P., M.J. Hossain, U.D. Parashar, M.M. Ali and T.G. Ksiazek et al.,20041020822087Joshi, R.M.,20182018Yu, M., E. Hansson, B. Shiell, W. Michalski, B.T. Eaton and L.F. Wang,19987917751780Wong, K.T., I. Grosjean, C. Brisson, B. Blanquier and M. Fevre-Montange et al.,200316321272137