Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2020.1659.1665Aeromonas hydrophila Infection in Tilapia (Oreochromis niloticus)]]>PhumkhachornParichat RattanachaikunsoponPongsak 1220202312Background and Objective: Antibiotics have been used to treat Aeromonas hydrophila infections in fish farming. However, their extensive uses can cause many negative effects including the development of drug-resistant bacterial strains. The main objective of this study was to find an alternative to antibiotics to inhibit A. hydrophila both in vitro and in vivo. Materials and Methods: A bacteriophage infecting A. hydrophila was isolated from a fish a pond water sample. It was classified based on its genome type studied by enzymatic digestion and morphology investigated by transmission electron microscopy. Its ability to control experimental A. hydrophila infection in tilapia (Oreochromis niloticus) was examined by feeding tilapia with fish diets supplemented with different titers of the bacteriophage. Results: A bacteriophage specific to Aeromonas hydrophila UR1 designated PAh4 was isolated and classified as a member of the family Myoviridae. When tilapia experimentally infected with A. hydrophila at the median lethal dose (3.16×105 CFU per fish) were fed the fish diets supplemented with the bacteriophage PAh4 at doses ranging from 105-108 PFU g1 of diet, the diets could reduce the mortality rate of infected tilapia in a dose-dependent manner. Conclusion: The bacteriophage PAh4 can be used as an alternative to antibiotics to control A. hydrophila infection in tilapia.]]>Janda, J.M. and S.L. Abbott,2010Aeromonas: Taxonomy, pathogenicity, and infection.]]>233573M.I. El-Barbary,2017Aeromonas hydrophila isolated from Oreochromis niloticus and Sparus aurata with multiple antibiotic resistance index.]]>17222234Laith, A.R. and M. Najiah,2013Aeromonas hydrophila: Antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell).]]>2013Jones, B.L. and M.H. Wilcox,199535453461Stratev, D. and O.A. Odeyemi,2016Aeromonas hydrophila isolated from different food sources: A mini-review.]]>9535544Liu, X., J.C. Steele and X.Z. Meng,2017223161169Okocha, R.C., I.O. Olatoye and O.B. Adedeji,20182018Principi, N., E. Silvestri and S. Esposito,20192019Perez-Sanchez, T., B. Mora-Sanchez and J.L. Balcazar,201826896903Phumkhachorn, P. and P. Rattanachaikunsopon, 2018Edwardsiella tarda. ]]>15117121Soliman, W.S., R.M. Shaapan, L.A. Mohamed and S.S. Gayed,20199190195Phumkhachorn, P. and P. Rattanachaikunsopon, 2014Siphoviridae bacteriophage specific to extended-spectrum b-lactamases-producing Escherichia coli. ]]>70604608Phumkhachorn, P. and P. Rattanachaikunsopon,2015Pseudomonas anguilliseptica infection in tilapia (Oreochromis niloticus).]]>6711651172Reed, L.J. and H. Muench,193827493497Miranda, C.D., F.A. Godoy and M.R. Lee,20182018Soothill, J.S., 199237258262Johnson, R.P., C.L. Gyles, W.E. Huff, S. Ojha, G.R. Huff, N.C. Rath and A.M. Donoghue,20089201215Doss, J., K. Culbertson, D. Hahn, J. Camacho and N. Barekzi,20172017Mateus, L., L. Costa, Y.J. Silva, C. Pereira, A. Cunha and A. Almeida,2014Vibrio in aquaculture.]]>424167173Ackermann, H.W.,1996141209218Laanto, E., L.R. Sundberg and J.K.H. Bamford,2011Flavobacterium columnare.]]>7778687872Kazimierczak, J., E.A. Wójcik, J. Witaszewska, A. Guziński and E. Górecka et al.,2019Aeromonas and Pseudomonas phages as a supportive tool for development of antibacterial treatment in aquaculture.]]>2019