Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2020.813.819TaechowisanThongchai SamsawatTipparat PuckdeeWinyou S. PhutdhawongWaya 62020236Background and Objective: Methyl 5-(hydroxymethyl)furan-2-carboxylate (MFC) was isolated as a major compound from Streptomyces zerumbet W14. This compound was evaluated the cytotoxicity and antibacterial activities. Materials and Methods: Isolation and structure elucidation of the bioactive compound were carried out using silica gel column chromatography and nuclear magnetic resonance. The cytotoxicity and antibacterial activities were reported. Results: MFC showed high antibacterial activity against Gram positive bacteria, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis and moderate activity against Gram negative bacteria, Escherichia coli, Salmonella Typhi, Serratia marcescens and Pseudomonas aeruginosa. This compound showed bacteriolytic effects on the tested microorganisms, causing cell wall and membrane damage by using the Sytox Green assay. It showed moderate cytotoxic activity on both normal cells, LLC-MK2 cells and L929 cells with IC50 values of >512.00 and 239.06 μg mL1, respectively. The strongest cytotoxicity was observed in HeLa and HepG2 cells with IC50 values of 64.00 and 102.53 μg mL1, respectively. Conclusion: The findings revealed that MFC affects selective cytotoxicity on cancer cells and Gram positive bacteria at a low concentration. It suggested that MFC has a potential usage as an alternative agents for treatments of some cancers and some bacterial infections.]]>Sardi, P., M. Saracchi, S. Quaroni, B. Petrolini, G.E. Borgonovi and S. Merli,1992Streptomyces strains from surface-sterilized roots.]]>5826912693Shimizu, M., Y. Nakagawa, Y. Sato, T. Furumai and Y. Igarashi et al.,2000Streptomyces sp. isolated from Rhododendron and its antifungal activity.]]>66360366Hasegawa, S., A. Meguro, M. Shimizu, T. Nishimura and H. Kunoh,2006207281Zhang, H.W., Y.C. Song and R.X. Tan,200623753771Gunatilaka, A.A.L.,200669509526Guo, B., Y. Wang, X. Sun and K. Tang,200844136142Staniek, A., H.J. Woerdenbag and O. Kayser,200837593Ryan, P.R., K. Germaine, A. Franks, D.J. Ryan and D.N. Dowling,200827819Verma, V.C., S.K. Gond, A. Mishra, A. Kumar, R.N. Kharwar and A.C. Gange,2009Azadirachta indica A. Juss.: Isolation, diversity and anti-microbial activity.]]>57749756Zhou, L., W. Dong, Y.D. Wang, K. Zhou and H.Y. Ma et al.,2016Cassia alata].]]>4126522654, (In Chinese)Liu, T., L. Liu, X. Jiang, X. Huang and J. Chen,2009Curvularia lunata, the causal agent of maize Curvularia leaf spot.]]>312227Che, J., C.J. Zheng, M.X. Song, Y.J. Bi and Y. Liu et al.,201423426435Ozdemir, A., M.D. Altıntop, Z. Canturk and Z.A. Kaplancikli,2015in vitro evaluation of furan-based chalcone derivatives as antimicrobial agents.]]>12607611Malladi, S., R.V. Nadh, K.S. Babu and P.S. Babu,20176345353Taechowisan, T., W. Puckdee and W.S. Phutdhawong,2019Streptomyces zerumbet, a novel species from Zingiber zerumbet (L.) Smith and isolation of its bioactive compounds.]]>9194219Coombs, J.T. and C.M.M. Franco,20036956035608Taechowisan, T., S. Chaisaeng and W.S. Phutdhawong,2017Streptomyces sp. BO-07: An endophyte in Boesenbergia rotunda (L.) Mansf A.]]>2813301346Taechowisan, T. and S. Lumyong,2003Zingiber officinale and Alpinia galanga against phytopathogenic fungi.]]>53291298CLSI.,20122012Suleiman, M.M., L.I. McGaw, V. Naidoo and J. Eloff,201076478NCCLS.,20002000Nikaido, H.,1994264382388Vaara, M.,199256395411Plesiat, P. and H. Nikaido,1992613231333Nikaido, H.,19961996pp: 29-47pp: 29-47Phutdhawong, W., S. Inpang, T. Taechowisan and W.S. Phutdhawong,20193510801085Langsrud, S. and G. Sundheim,199681411418Roth, B.L., M. Poot, S.T. Yue and P.J. Millard,19976324212431Taechowisan, T., S. Suttichokthanakorn and W.S. Phutdhawong,2018Zingiber cassumunar Roxb.]]>8121127Oussalah, M., S. Caillet and M. Lacroix,2006Escherichia coli O157: H7 and Listeria monocytogenes.]]>6910461055Saad, N.Y., C.D. Muller and A. Lobstein,201328269279Prinsep, M.R., J.W. Blunt and M.H. Munro,1994Hymeniacidon hauraki.]]>5715571559Wang, J., H. Zhang, X. Yang, Y. Zhou, H. Wang and H. Bai,2008Streptomyces sp. HS-HY-071.]]>61623626Suthindhiran, K. and K. Kannabiran,2013in vitro and in silico analysis-pharmacological study.]]>8118