Shafiee Rasool
Faculty of Science, Isfahan Medical University, Iran
Nahvi Iraj
Faculty of Science, Isfahan Medical University, Iran
Emtiazi Giti
Faculty of Science, Isfahan Medical University, Iran
ABSTRACT
Production of trehalose by an isolated starch assimilating yeast Cryptococcus aerius under some conditions such as different concentration of carbon source, temperature stress, oxygen limitation and various nitrogen sources was investigated. Trehalose production was favorable in a medium contained 1% starch; in a time course about 72 h at 30°C. It was proved that the starch degradation rate and reducing sugars concentration in medium were the factors that controlled trelahose accumulation in all conditions. Cultivation of C. aerius at 37°C was a stress condition for this yeast, but because of low starch degradation rate at this temperature it was not an optimum condition for trehalose accumulation. Due to high rate of starch degradation and assimilation by C. aerius in the presence of inorganic nitrogen source, trehalose accumulation rate improved relative to using complex organic nitrogen source such as yeast extract. These results indicate that trehalose production can be conducted efficiently by using starch as carbon source and a yeast like C.aerius.
PDF References
How to cite this article
Shafiee Rasool, Nahvi Iraj and Emtiazi Giti, 2005. Trehalose Production by a Starch Assimilating Yeast Cryptococcus aerius. Biotechnology, 4: 279-283.
DOI: 10.3923/biotech.2005.279.283
URL: https://scialert.net/abstract/?doi=biotech.2005.279.283
DOI: 10.3923/biotech.2005.279.283
URL: https://scialert.net/abstract/?doi=biotech.2005.279.283
REFERENCES
- MacFaddin, J.F., 2000. Biochemical Tests for Identification of Medical Bacteria. 3rd Edn., Lippincott Williams & Wilkins, Pennsylvania, United States, ISBN: 0-683-05318-3, Pages: 912.
Direct Link - Abouzied, M.M. and C.A. Reddy, 1986. Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae. Applied Environ. Microbiol., 52: 1055-1059.
PubMedDirect Link - Wilson, J.J. and W.M. Ingledew, 1982. Isolation and characterization of Schwaniomyces alluvius amylolytic enzymes. Applied Environ. Microbiol., 44: 301-307.
PubMed