Hamid M. M. Sadeghi
Department of Pharmacology, Faculty of Pharmacy, Medical University of Isfahan, Iran
M. Rabbani
Department of Pharmacology, Faculty of Pharmacy, Medical University of Isfahan, Iran
A. Jafarian D.
Department of Pharmacology, Faculty of Pharmacy, Medical University of Isfahan, Iran
T. Ghafghazi
Department of Pharmacology, Faculty of Pharmacy, Medical University of Isfahan, Iran
H. Najafzzadeh V.
Department of Pharmacology, Faculty of Pharmacy, Medical University of Isfahan, Iran
ABSTRACT
The present study was conducted to create mutations in this motif for further investigation of its function. By using nested PCR two mutations were produced, which would translate to DRY and DKH. The PCR products as well as the PGEM3Z vector were digested using NcoI and EcoRI restriction enzymes and were ligated and transformed to E. coli HB101 cells. The obtained colonies were analyzed for the presence of the inserts using suitable restriction enzymes. The obtained plasmids have the advantage of having restriction sites, which would not interfere with further cloning and expression of this receptor using mammalian expression vectors.
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How to cite this article
Hamid M. M. Sadeghi, M. Rabbani, A. Jafarian D., T. Ghafghazi and H. Najafzzadeh V., 2005. Site Directed Mutagenesis of V2 Vasopressin Receptor and its Cloning Using PGEM3Z Vector. Biotechnology, 4: 284-287.
DOI: 10.3923/biotech.2005.284.287
URL: https://scialert.net/abstract/?doi=biotech.2005.284.287
DOI: 10.3923/biotech.2005.284.287
URL: https://scialert.net/abstract/?doi=biotech.2005.284.287
REFERENCES
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