Masoud Soleimani
School Of Medical Science, Tarbiat Modaress University, Tehran, Iran
Hossein Mozdarani
School Of Medical Science, Tarbiat Modaress University, Tehran, Iran
Ali Akbar Pourfathollah
School Of Medical Science, Tarbiat Modaress University, Tehran, Iran
Yousef Mortazavi
Department of Pathology, Zanjan Medical University, Zanjan, Iran
Kamran Alimoghaddam
Bone Marrow Transplantation Center, Tehran, Iran
Mahin Nikogoftar
Iranian Blood Transfusion Center, Terhan, Iran
Zahra Zonobi
Shahid Behesti Medical Science University, Tehran, Iran
Abbas Hajifathali
Iranian Blood Transfusion Organization Research Center, Terhan, Iran
ABSTRACT
Many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. The proliferation and expansion of Human Hematopoietic Stem Cells (HSC) in ex vivo culture was examined with the goal of generating a suitable protocol for expanding HSC for patient transplantation. Using primary fetal liver cells, we established a serum-free culture system to expand human primitive stem/progenitors cells. Non enriched cord blood CD34+ cells were cultured on a monolayer of mouse primary fetal liver cells in the presence of trombopoietin, Flt3/Flk2 ligand and/or stem cell factor, IL-6 and IL-3 under serum-free conditions. After 1 or 2 weeks of culture, cells were examined for clonogenic progenitors and percentage of CD34+, CD38 cells. In the presence of trombopoietin, Flt3/Flk2 ligand and stem cell factor, fetal liver cells supported more than a 10- to 20-fold expansion of CD34+ cells. In addition, CFU-C assay were expanded more than 5 and 10 fold after 1 and 2 weeks of culture, respectively. These results strongly suggest that fetal liver cells may be a suitable feeder layer for expansion of hematopoietic progenitors from umbilical cord blood in vitro.
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How to cite this article
Masoud Soleimani, Hossein Mozdarani, Ali Akbar Pourfathollah, Yousef Mortazavi, Kamran Alimoghaddam, Mahin Nikogoftar, Zahra Zonobi and Abbas Hajifathali, 2005. A Co-culture System for Expansion of Nonenriched Cord Blood Stem/Progenitor Cells. Biotechnology, 4: 310-315.
DOI: 10.3923/biotech.2005.310.315
URL: https://scialert.net/abstract/?doi=biotech.2005.310.315
DOI: 10.3923/biotech.2005.310.315
URL: https://scialert.net/abstract/?doi=biotech.2005.310.315
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