Rouzbeh Chegeni
Tarbiat Modarres University, Tehran, Iran
Bahram Kazemi
Shaheed Beheshti Unversity of Medical Science, Tehran, Iran
Abbas Hajifathali
College of Medicine, Shaheed Beheshti Unversity of Medical Science, Tehran, Iran
Aliakbar Pourfathollah
Iranian Blood Transfusing Organization, Iran
Ghasem Rastegar Lari
Iranian Comprehensive Hemophilia Center, Iran
Minoo Ahmadi
Iranian Blood Transfusing Organization, Iran
Mohammadreza Tabatabaie
Iranian Blood Transfusing Organization, Iran
Yadollah Mehrabi
Shaheed Beheshti Unversity of Medical Science, Tehran, Iran
ABSTRACT
Human coagulation factor V along with coagulation factor serves as a cofactor in thrombin formation by coagulation factor X. Generation of thrombin leads to fibrin and clot formation. In this study 45 patients were selected with venous thrombosis and evaluated first by the coagulation test for the presence of activated protein C (APC) resistance and then evaluated genetically by restriction enzyme analysis for the presence of factor V Leiden. 9(21%) patients had activated protein C resistance by the modified coagulation assay. When analyzed by restriction analysis, 7 (17%) had the factor V Leiden mutation. The present study shows that factor V Leiden has a lower frequency in the Iranian patients with venous thrombosis compared to the western societies.
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How to cite this article
Rouzbeh Chegeni, Bahram Kazemi, Abbas Hajifathali, Aliakbar Pourfathollah, Ghasem Rastegar Lari, Minoo Ahmadi, Mohammadreza Tabatabaie and Yadollah Mehrabi, 2005. Detection of the Factor V Leiden Mutation in Iranian Patients with Venous Thrombosis. Biotechnology, 4: 76-78.
DOI: 10.3923/biotech.2005.76.78
URL: https://scialert.net/abstract/?doi=biotech.2005.76.78
DOI: 10.3923/biotech.2005.76.78
URL: https://scialert.net/abstract/?doi=biotech.2005.76.78
REFERENCES
- Zoller, B., P. Svensson, X. He and B. Dahlback, 1994. Identification of thesame factor V gene mutation in 47 out of 50 thrombosis-prone families with inherited resistance to activated protein C. J. Clin. Invest., 94: 2521-2521.
Direct Link - Bertina, R., B. Koeleman, T. Koster, F. Rosendaal and R. Dirven et al., 1994. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature, 369: 64-67.
CrossRef - Voorberg, J., J. Roelse, R. Koopman, H. Buller, F. Berends and J.T. Cate et al., 1994. Association of idiopathic venous thromboembolism with single point-mutation at Arg506 of factor V. Lancet, 343: 1535-1536.
PubMed - Greengard, J., X. Sun, X. Xu, J. Fernandez, J. Griffin and B. Evatt, 1994. Activated protein C resistance caused by Arg506Gln mutation in factor Va. Lancet, 343: 1361-1362.
Direct Link - Dahlback, B., 1994. Physiological anticoagulation: Inherited resistance to activated protein C and venous thromboembolism. J. Clin. Invest., 94: 923-927.
CrossRef - Jorquera, J., J. Montoro, M. Fernandez, J.A. Aznar and J. Aznar, 1994. Modified test for activated protein C resistance. Lancet, 344: 1162-1163.
PubMed - Nicholaes, G.A.F. and B. Dahlback, 2003. Activated Protein C resistance (FV Leiden) and thrombosis: Factor V mutations causing hypercoagulable states. Hematol. Oncol. Clin. N. Am., 17: 37-61.
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