Wei Ye
College of Shanghai Medical Instrumentation, University of Shanghai for Science and Technology, Shanghai, China
Junliang Chen
College of Food and Bioengineering, Henan University of Science and Technology, Henan, China
Guicheng Huo
Key Lab of Dairy Science, Ministry of Education, Northeast Agricultural University, 150030, Harbin, China
ABSTRACT
The plasmids content of nine different S. thermophilus strains of KLDS were identified. The strains KLDS3.0201, KLDS3.0203 and KLDS3.0208 isolated from the commercial yoghurt were free of plasmid. The strains S9, S11, S14 and S16 which were isolated from the traditional fermented milk of china that all contained different plasmids and the strains S10 and S15 were free of plasmid. The plasmids of the S14 were analyzing, plasmid rolling circle replication initiator protein has been sequenced. This part has the same sequence with the LMD-9 plasmid 2. Biogenic Amines were determined by multiplex PCR the related partial genes which were histidine decarboxylase (hdc) gene, ornithine decarboxylase (odc) gene and tyramine decarboxylase (tdc) gene. There was no relative amplification gene in these nine strains. They were safe for the production of the yoghurt.
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How to cite this article
Wei Ye, Junliang Chen and Guicheng Huo, 2013. Content of Plasmids and Biogenic Amines Identification of Streptococcus thermophilus of Fermented Milk. Biotechnology, 12: 109-112.
DOI: 10.3923/biotech.2013.109.112
URL: https://scialert.net/abstract/?doi=biotech.2013.109.112
DOI: 10.3923/biotech.2013.109.112
URL: https://scialert.net/abstract/?doi=biotech.2013.109.112
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