Yuan Kping
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, 430223, Wuhan, People`s Republic of China
Mao Tao
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, 430223, Wuhan, People`s Republic of China
Su Yinbin
College of Animal Science, Yangzte University, 434000, Jingzhou, People`s Republic of China
Huang Jian
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, 430223, Wuhan, People`s Republic of China
ABSTRACT
A new aerobic denitrifying bacterium strain B6-2, which was isolated from pond sediment, had showed the great abilities in denitrification. It can remove as high as 284.6 mg L-1 NO3--N and 187.54 mg L-1 NO2--N within 24 h. The biochemical and phylogenetic analysis indicate that, the genus of the bacterium was identified as Pseudomonas and closed to mendocina species. In this study, several experiments were conducted to detect the nitrate removal response at different carbon sources, C/N ratios, temperatures and shaking speeds and the production of N2 was verified by gas chromatography technique. Nearly 40% of removed NO3>--N was totally deoxidized to nitrogen gas under optimum conditions (citrate as carbon source, C/N 10, 30EC, 120 r min-1). The results indicate that the strain has highly aerobic denitrification ability and this reveals high promise for future application.
PDF References
How to cite this article
Yuan Kping, Mao Tao, Su Yinbin and Huang Jian, 2013. Aerobic Denitrification by Pseudomonas sp. B6-2 Isolated from Pond
Sediment. Biotechnology, 12: 140-145.
DOI: 10.3923/biotech.2013.140.145
URL: https://scialert.net/abstract/?doi=biotech.2013.140.145
DOI: 10.3923/biotech.2013.140.145
URL: https://scialert.net/abstract/?doi=biotech.2013.140.145
REFERENCES
- Ferguson, S.J., 1994. Denitrification and its control. Antonie Leeuwenhoek, 66: 89-110.
CrossRefDirect Link - Jae, Y.P. and J.Y. Young, 2009. Biological nitrate removal in industrial wastewater treatment: Which electron donor we can choose. Applied Microbiol. Biotechnol., 82: 415-429.
CrossRef - Jang, A.M., M. Bum, S. Kim, Y. Ahn, I.S. Kim and P.L. Bishop, 2005. Assessment of characteristics of biofilm formed on autotrophic denitrification. J. Microbiol. Biotechnol., 15: 455-460.
Direct Link - Joo, H.S., M. Hirai and M. Shoda, 2005. Nitrification and denitrification in high-strength ammonium by Alcaligenes faecalis. Biotechnol. Lett., 27: 773-778.
CrossRefDirect Link - Kariminiaae-Hamedaani, H.R., K. Kanda and F. Kato, 2004. Denitrification activity of the bacterium Pseudomonas sp. ASM-2-3 isolated from the Ariake Sea tideland. J. Biosci. Bioeng., 97: 39-44.
CrossRef - Khardenavis, A.A., A. Kapley and H.J. Purohit, 2007. Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Applied Microbiol. Biotechnol., 77: 403-409.
CrossRef - Kim, M., S.Y. Jeong, S.J. Yoon, S.J. Cho and Y.H. Kim et al., 2008. Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios. J. Biosci. Bioeng., 106: 498-502.
CrossRef - McAdam, E.J. and S.J. Judd, 2006. A review of membrane bioreactor potential for nitrate removal from drinking water. Desalination, 196: 135-148.
CrossRef - Ovez, B., S. Ozgen and M. Yuksel, 2006. Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source. Process Biochem., 41: 1539-1544.
CrossRef - Qi, Z., T. Shinji, M. Shuichiro, S. Phisit, K. Ampin and A. Kenji, 2007. Screening and characterization of bacteria that can utilize ammonium and nitrite ions simultaneously under controlled cultural conditions. J. Biosci. Bioeng., 103: 185-191.
CrossRefDirect Link - Rajakumar, S., P.M. Ayyasamy, K. Shanthi, P. Thavamani, P. Velmurugan, Y.C. Song and P. Lakshmanaperumalsamy, 2008. Nitrate removal efficiency of bacterial consortium (Pseudomonas sp. KW1 and Bacillus sp. YW4) in synthetic nitrate-rich water. J. Hazardous Mater., 157: 553-563.
CrossRef - Su, J.J., K.S. Yeh and P.W. Tseng, 2006. A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan. Curr. Microbiol., 53: 77-81.
CrossRef - Takaya, N., M.A.B. Catalan-Sakairi, Y. Sakaguchi, I. Kato, Z. Zhou and H. Shoun, 2003. Aerobic denitrifying bacteria that produce low levels of nitrous oxide. Applied Environ. Microbiol., 69: 3152-3157.
CrossRef - Taylor, S.M., Y. He, B. Zhao and J. Huang, 2009. Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL. J. Environ. Sci., 21: 1336-1341.
CrossRef - Wang, P., X. Li, M. Xiang and Q. Zhai, 2007. Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis. J. Biosci. Bioeng., 103: 563-567.
CrossRef - Zhang, J., P. Wu, B. Hao and Z. Yu, 2011. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresour. Technol., 102: 9866-9869.
CrossRefDirect Link