Hui Fan
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
Yu Mi
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
Junfeng Hui
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
Jianjun Deng
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
Pei Ma
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
Daidi Fan
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi`an, 710069, Shaanxi, China
ABSTRACT
Human-like Collagen (HLC)/Nano-hydroxyapatite (nHA) porous scaffold was constructed for the culture of cartilages. The cellular structure of scaffold and pore size were examined through Scanning Electron Microscopy (SEM). The pores with diameter ranging from 100 to 300 μm distributed evenly in the scaffolds and these pores interpenetrated and interconnected with each other. Rabbit ear cartilages were separated using digestion method and then cultured on the HLC/nHA scaffolds to evaluate the compatibility and cytocompatibility of scaffolds. After cultured for 14 days, cartilages maintained chondrocytic spherical morphology and infiltrated into porous scaffolds. This demonstrated excellent cytocompatibility and compatibility of HLC/nHA scaffolds. HLC/nHA porous scaffolds manifested great promising prospect in cartilage tissue engineering.
PDF References
How to cite this article
Hui Fan, Yu Mi, Junfeng Hui, Jianjun Deng, Pei Ma and Daidi Fan, 2013. Cytocompatibility of Human-like Collagen/nano-hydroxyapatite Porous Scaffolds Using Cartilages. Biotechnology, 12: 99-103.
DOI: 10.3923/biotech.2013.99.103
URL: https://scialert.net/abstract/?doi=biotech.2013.99.103
DOI: 10.3923/biotech.2013.99.103
URL: https://scialert.net/abstract/?doi=biotech.2013.99.103
REFERENCES
- Alves da Silva, M.L., A. Crawford, J.M. Mundy, V.M. Correlo and P. Sol et al., 2010. Chitosan/polyester-based scaffolds for cartilage tissue engineering: Assessment of extracellular matrix formation. Acta Biomaterialia, 6: 1149-1157.
CrossRef - Donzelli, E., A. Salvade, P. Mimo, M. Vigano and M. Morrone et al., 2007. Mesenchymal stem cells cultured on a collagen scaffold: In vitro osteogenic differentiation. Arch Oral Biol., 52: 64-73.
CrossRef - Guo, J.Q., Y.E. Luo, D.D. Fan, P.F. Gao, X.X. Ma and C.H. Zhu, 2010. Analysis of metabolic products by response surface methodology for production of human-like Collagen II. Chinese J. Chem. Eng., 18: 830-836.
CrossRef - Hua, X.F., D.D. Fan, Y.E. Luo, X. Zhang and H. Shi et al., 2006. Kinetics of high cell density fed-batch culture of recombinant Escherichia coli producing human-like collagen. Chinese J. Chem. Eng., 14: 242-247.
CrossRef - Kang, J.Y., C.W. Chung, J.H. Sung, B.S. Park and J.Y. Choi et al., 2009. Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes. Int. J. Pharm., 369: 114-120.
CrossRefDirect Link - Ma, L., C.Y. Gao, Z.W. Mao, J. Zhou, J.C. Shen, X.Q. Hu and C. Han, 2003. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials, 24: 4833-4841.
CrossRef - Wang, Y.Y., L. Liu and S.R. Guo, 2010. Characterization of biodegradable and cytocompatible nano-hydroxyapatite/polycaprolactone porous scaffolds in degradation in vitro. Polym. Degrad. Stabil., 95: 207-213.
CrossRefDirect Link - Zhao, F., Y. Yin, W.W. Lu, J.C. Leong and W. Zhang et al., 2002. Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds. Biomaterials, 23: 3227-3234.
CrossRef