S. A. Leigh
USDA, ARS Mid-South Area Poultry Research Unit, Mississippi State, MS 39762, USA
J. D. Evans
USDA, ARS Mid-South Area Poultry Research Unit, Mississippi State, MS 39762, USA
ABSTRACT
Mycoplasma gallinarum colonizes poultry as well as mammals, but is considered to have a commensal relationship with its hosts. Though unable to cause poultry disease by its self, reports have been published suggesting a synergism during mixed infections between M. gallinarum and respiratory viruses or their vaccine strains that can precipitate airsacculitis. Currently, little research is being done on M. gallinarum and little is known about its carrier rate in chickens and other poultry. Two primer sets were tested for their ability to detect M. gallinarum using real-time PCR. One set published by Lauerman amplifies a fragment from the M. gallinarum 16S ribosomal DNA sequence. The other set (101-2) was developed in this laboratory and amplifies a short segment of DNA that appears to be unique to some strains of M. gallinarum. The Lauerman primer set is specific for M. gallinarum and has a detection limit of 100 genomes. The 101-2 primer set is specific for some strains of M. gallinarum, although, it is 100-fold less sensitive than the Lauerman primer set. The 101-2 primer set appears to be unsuited for M. gallinarum detection, but it does provide a method of differentiating M. gallinarum strains by PCR. These primer sets provide a means to rapidly determine the M. gallinarum carrier status of flocks by real-time PCR and will help in identifying M. gallinarum in mixed infections.
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How to cite this article
S. A. Leigh and J. D. Evans, 2009. Detection of Mycoplasma gallinarum by Real-Time PCR. International Journal of Poultry Science, 8: 108-111.
DOI: 10.3923/ijps.2009.108.111
URL: https://scialert.net/abstract/?doi=ijps.2009.108.111
DOI: 10.3923/ijps.2009.108.111
URL: https://scialert.net/abstract/?doi=ijps.2009.108.111
REFERENCES
- Bencina, D., D. Dorrer and T. Tadina, 1987. Mycoplasma species isolated from six avian species. Avian Pathol., 16: 653-664.
CrossRefPubMedDirect Link - Bradbury, J.M., 1984. Avian mycoplasma infections: Prototype of mixed infections with mycoplasmas, bacteria and viruses. Ann. Microbiol. (Paris), 135A: 83-89.
PubMed - Branton, S.L., S.M.D. Bearson, B.L. Bearson, W.R. Maslin and S.D. Collier et al., 2003. Mycoplasma gallinarum infection in commercial layers and onset of fatty liver hemorrhagic syndrome. Avian Dis., 47: 458-462.
Direct Link - Dovc, P., D. Bencina and I. Zajc, 1991. Genotypic heterogeneity among strains of Mycoplasma gallinarum. Avian Pathol., 20: 705-711.
PubMed - Kleven, S.H., C.S. Eidson and O.J. Fletcher, 1978. Airsacculitis induced in broilers with a combination of Mycoplasma gallinarum and respiratory viruses. Avian Dis., 22: 707-716.
Direct Link - Lauerman, L.H., A.R. Chilina, A.R. Closser and D. Johansen, 1995. Avian mycoplasma identification using polymerase chain reaction amplicon and restriction fragment length polymorphism analysis. Avian Dis., 39: 804-811.
PubMedDirect Link - Lauerman, L.H., S.M. Shah, J.C. Williams, C.M. Corsiglia and R.J. Herring, 1993. Immunoglobulin receptors used in avian Mycoplasma identification. Avian Dis., 37: 1080-1084.
Direct Link - Power, J. and F.T. Jordan, 1976. A comparison of the virulence of three strains of Mycoplasma gallisepticum and one strain of Mycoplasma gallinarum in chicks, turkey poults, tracheal organ cultures and embryonated fowl eggs. Res. Vet. Sci., 21: 41-46.
PubMed - Robinson, T.D., M.H. Williams and D.A. Haig, 1968. The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. J. Gen. Microbiol., 54: 33-46.
CrossRefDirect Link - Robinson, T.D. and Z. Dinter, 1968. Mycoplasma host specificity in the light of strains isolated from pigs. J. Gen. Microbiol., 53: 221-229.
CrossRefDirect Link - Singh, K.C. and P.K. Uppal, 1987. Isolation of Mycoplasma gallinarum from sheep. Vet. Rec., 120: 464-464.
Direct Link - Wan, X., S.L. Branton, L.A. Hanson and G.T. Pharr, 2004. Identification and initial characterization of a putative Mycoplasma gallinarum leucine aminopeptidase gene. Curr. Microbiol., 48: 32-38.
PubMedDirect Link