G. Dhinakar Raj
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
T.M. Chozhavel Rajanathan
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
K. Kumanan
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
S. Elankumaran
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
ABSTRACT
Expression profile of chicken six Toll-like Receptor (TLR) mRNAs were analyzed in the heterophils, lungs, liver, spleen, duodenum, caecal tonsils and kidneys of 12-weeks old indigenous village chicken in India, using reverse transcription polymerase chain reaction (RT-PCR). TLR 2 type 1 mRNA was expressed in lungs, liver, spleen, duodenum and caecal tonsils. TLR2 type 2 mRNA was expressed only in the lungs. TLR 3 mRNA was expressed in lungs, liver, spleen and caecal tonsils. TLR 4 mRNA was expressed only in lungs, liver and spleen. TLR 5 and TLR 7 mRNAs were expressed in all the tissues examined. With respect to tissues, heterophils and lungs expressed all the TLR mRNAs examined while kidneys expressed only TLR 5 and TLR 7 mRNAs. All the TLR amplified PCR products were partially sequenced and showed high homologies with the available chicken (commercial) TLR gene sequences. There could be a possibility of correlation between TLR mRNA expressions with higher levels of innate immunity seen in indigenous village breeds of chickens.
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How to cite this article
G. Dhinakar Raj, T.M. Chozhavel Rajanathan, K. Kumanan and S. Elankumaran, 2009. Expression Profile of Toll-Like Receptor mRNA in an Indigenous Aseel Breed of Chicken in India. International Journal of Poultry Science, 8: 651-655.
DOI: 10.3923/ijps.2009.651.655
URL: https://scialert.net/abstract/?doi=ijps.2009.651.655
DOI: 10.3923/ijps.2009.651.655
URL: https://scialert.net/abstract/?doi=ijps.2009.651.655
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