Samuel Besong
Department of Human Ecology, Delaware State University, 1200 North DuPont HWY, John Price Bldg RM #103, Dover, DE 19901
Celestine Fosung
Department of Human Ecology, Delaware State University, 1200 North DuPont HWY, John Price Bldg RM #103, Dover, DE 19901
Ayasha Jones
Department of Human Ecology, Delaware State University, 1200 North DuPont HWY, John Price Bldg RM #103, Dover, DE 19901
Vincent Fondong
Department of Biological Science, Delaware State University, 1200 North DuPont HWY, Dover, DE 19901
ABSTRACT
Pathogenesis of liver steatosis in laying hen remains poorly understood. However, it has been suggested that regulation of the expression of Stearoyl-Coenzyme A Desaturase-1 (SCD-1) may alter hepatic lipid metabolism and prevent liver steatosis in laying hens. SCD-1 is an integral membrane protein that catalyzes the biosynthesis of monounsaturated fatty acids required for the synthesis of triglycerides, cholesterol esters and phospholipids. This study was conducted to determine whether Njangsa Oil Seed (NOS) (Ricinodendron heudelotii) which is rich in polyunsaturated fatty acids, specifically omega-3 fatty acids can regulate the expression of SCD-1 gene and lower hepatic lipid accumulation in laying hens. The experiment was carried out with seventy-two white leghorn hens (24 months old) which were randomly assigned to three layer diet supplemented with 0%, 10% or 20% NOS for 6 weeks. The mRNA levels in SCD-1 gene was quantified using forward and reverse primers specific to chicken SCD-1 in real-time reverse transcription-PCR (RT-PCR) technique with SYBER Green detection. Histological studies with liver tissues were performed with oil red stain and electron transmission microscope. Results indicate that NOS supplementation at 10% level tended to increase (p>0.05) SCD-1 mRNA expression in hens. Histological examination revealed micro-vesicular fat accumulation in the liver of hen fed NOS diets, indicating that supplementation of NOS in the diet may not prevent development of fatty liver. Results from this study suggest that NOS supplementation may regulate genes involve in lipid biosynthesis but may not prevent liver steatosis.
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How to cite this article
Samuel Besong, Celestine Fosung, Ayasha Jones and Vincent Fondong, 2011. Effect of Njangsa Oil Seeds (Ricinodendron heudelitii) on Expression of Stearoyl-coenzyme a Desaturase in Laying Hens. International Journal of Poultry Science, 10: 257-265.
DOI: 10.3923/ijps.2011.257.265
URL: https://scialert.net/abstract/?doi=ijps.2011.257.265
DOI: 10.3923/ijps.2011.257.265
URL: https://scialert.net/abstract/?doi=ijps.2011.257.265
REFERENCES
- Cohen, P., M. Miyazaki, N.D. Socci, A.H. Greenberg and W. Liedtke et al., 2002. Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science, 297: 240-243.
PubMedDirect Link - Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
CrossRefPubMedDirect Link - Gebauer, S.K., T.L. Psota, W.S. Harris and P.M. Kris-Etherton, 2006. n-3 Fatty acid dietary recommendations and food sources to achieve essentiality and cardiovascular benefits. Am. J. Clin. Nutr., 83: S1526-S1535.
Direct Link - Harris, W.S. and W.E. Connor, 1980. The effects of salmon oil upon plasma lipids, lipoproteins and triglyceride clearance. Trans. Assoc. Am. Physicians, 93: 148-155.
PubMed - Harris, W.S., B.E. Hustvedt, E. Hagen, M.H. Green, G. Lu and C.A. Drevon, 1997. N-3 fatty acids and chylomicron metabolism in the rat. J. Lipid Res., 38: 503-515.
Direct Link - Huuskonen, J., V.M. Olkkonen, M. Jauhiainen and C. Ehnholm, 2001. The impact of Phospholipid Transfer Protein (PLTP) on HDL metabolism. Atherosclerosis, 155: 269-281.
PubMed - Jordal, A.E., B.E. Torstensen, S. Tsoi, D.R. Tocher, S.P. Lall and S.E. Douglas, 2005. Dietary rapeseed oil affects the expression of genes involve in hepatic lipid metabolism in atlantic salmon (Salmo sala L.). J. Nutr., 135: 2355-2361.
PubMed - Khan, S., A.M. Minihane, P.J. Talmud, J.W. Wright, M.C. Murphy, C.M. Williams and B.A. Griffin, 2002. Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype. J. Lipid Res., 43: 979-985.
PubMed - Krauss, R.M., R.H. Eckel, B. Howard, L.J. Appel and S.R. Daniels et al., 2000. AHA dietary guidelines: Revision 2000: A statement for healthcare professionals from the nutrition committee of the American heart association. Circulation, 2: 2284-2299.
PubMedDirect Link - Kris-Etherton, P.M., D.S. Taylor, S. Yu-Poth, P. Huth and K. Moriarty et al., 2000. Polyunsaturated fatty acids in the food chain in the United States. Am. J. Clin. Nutr., 71: 179s-188s.
PubMed - Miyazaki, M., W.C. Man and J.M. Ntambi, 2001. Targeted disruption of stearoyl-CoA desaturase1 gene in mice causes atrophy of sebaceous and meibomian glands and depletion of wax esters in the eyelid. J. Nutr., 131: 2260-2268.
PubMed - Miyazaki, M. and J.M. Ntambi, 2003. Role of stearoyl-coenzyme a desaturase in lipid metabolism. Prostaglandins Leukot Essent Fatty Acids, 68: 113-121.
CrossRefPubMedDirect Link - NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Direct Link - Ntambi, J.M., 1999. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. J. Lipid Res., 40: 1549-1558.
Direct Link - Richards, G.P., M.A. Watson and D.H. Kingsley, 2004. A SYBR green, real-time RT-PCR method to detect and quantitate Norwalk virus in stools. J. Virol. Methods, 116: 63-70.
PubMed - Richards, G.P., M.A. Watson, R.L. Fankhauser and S.S. Monroe, 2004. Genogroup I and II Noroviruses detected in stool samples by real-time reverse transcription-PCR using highly degenerate universal primers. Appl. Environ. Microbiol., 70: 7179-7188.
CrossRefDirect Link