M.H. Tamzil
Faculty of Animal Sciences, Mataram University, Jl. Majapahit Mataram, Lombok, West Nusa Tenggara-83125, Indonesia
R.R. Noor
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
P.S. Hardjosworo
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
W. Manalu
Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
C. Sumantri
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
ABSTRACT
Hematological responses in chicken with different HSP 70 genotypes to acute heat stress were studied using 28 kampong chickens, 24 Arabic chickens and 4 commercial chickens. The experimental chickens were selected randomly from a group of chickens with HSP 70 genotypes identified and were exposed to ambient temperature (40°C) for 0.5, 1.0 and 1.5 h. Results showed that erythrocyte, hematocrit and leukocyte in all chicken lines decreased in response to acute heat stress with the highest decrease in commercial chickens, followed by Arabic and kampong chickens. Regardless of acute heat stress exposure, there was no significant difference in erythrocyte, hemoglobin, leukocyte, heterophil, eosinophil, basophil, lymphocyte, monocyte and heterophil/lymphocyte ratio in all chicken lines studied. Arabic and commercial chickens had lower hematocrit as compared to kampong chickens. However, acute heat stress increased the percentage of heterophil, basophil, lymphocyte and heterophil/lymphocyte ratio without affecting eosinophil and monocyte. It was found that there was no interaction between lines of chicken and acute heat-stress exposure on the hematological parameters measured. The lowest percentage of lymphocytes was found in chickens with DD HSP 70 genotype while the highest was found in chickens with AD genotype. The results indicated that there was a relationship of heat resistance or tolerance to lymphocyte expression. Chickens that were the most tolerant to acute heat stress had the highest lymphocyte percentage (AD genotype) whereas those that were the least tolerant had the lowest lymphocyte percentage (DD genotype).
PDF References Citation
How to cite this article
M.H. Tamzil, R.R. Noor, P.S. Hardjosworo, W. Manalu and C. Sumantri, 2014. Hematological Response of Chickens with Different Heat Shock Protein 70 Genotypes to Acute Heat Stress. International Journal of Poultry Science, 13: 14-20.
DOI: 10.3923/ijps.2014.14.20
URL: https://scialert.net/abstract/?doi=ijps.2014.14.20
DOI: 10.3923/ijps.2014.14.20
URL: https://scialert.net/abstract/?doi=ijps.2014.14.20
REFERENCES
- Ajakaiye, J.J., J.O. Ayo and S.A. Ojo, 2010. Effects of heat stress on some blood parameters and egg production of Shika Brown layer chickens transported by road. Biol. Res., 43: 183-189.
CrossRefDirect Link - Al-Aqil, A. and I. Zulkifli, 2009. Changes in heat shock protein 70 expression and blood characteristics in transported broiler chickens as affected by housing and early age feed restriction. Poult. Sci., 88: 1358-1364.
CrossRefDirect Link - Al-Fataftah, A.R.A. and Z.H.M. Abu-Dieyeh, 2007. Effect of chronic heat stress on broiler performance in Jordan. Int. J. Poult. Sci., 6: 64-70.
CrossRefDirect Link - Al-Ghamdi, Z.H., 2008. Effects of commutative heat stress on immune responses in broiler chickens reared in closed system. Int. J. Poult. Sci., 7: 964-968.
Direct Link - Altan, O., A. Altan, M. Cabuk and H. Bayraktar, 2000. Effects of heat stress on some blood parameters in broilers. Turk. J. Vet. Anim. Sci., 24: 145-148.
Direct Link - Cooper, M.A. and K.W. Washburn, 1998. The relationships of body temperature to weight gain, feed consumption and feed utilization in broilers under heat stress. Poult. Sci., 77: 237-242.
CrossRefPubMedDirect Link - Davis, A.K., D.L. Maney and J.C. Maerz, 2008. The use of leukocyte profiles to measure stress in vertebrates: A review for ecologists. Funct. Ecol., 22: 760-772.
CrossRefDirect Link - Fumihito, A., T. Miyake, M. Takada, R. Shingu and T. Endo et al., 1996. Monophyletic origin and unique dispersal patterns of domestic fowls. Proc. Natl. Acad. Sci. USA., 93: 6792-6795.
PubMedDirect Link - Gaviol, H.C.T., E. Gasparino, A.J. Prioli and M.A. Soares, 2008. Genetic evaluation of the HSP70 protein in the Japanese quail (Coturnix japonica). Genet. Mol. Res., 7: 133-139.
PubMed - Iskandar, S., H. Resnawati and T. Pasaribu, 2003. Growth and carcass responses of three lines of local chickens and its crossing to dietary lysine and methionine. Proceedings of the 3rd International Seminar on Tropical Animal Production and Total Management of Local Resources, October 15-16, 2003, Gajah Mada University.
- Lin, H., H.F. Zhang, R. Du, X.H. Gu, Z.Y. Zhang, J. Buyse and E. Decuypere, 2005. Thermoregulation responses of broiler chickens to humidity at different ambient temperatures. II. Four weeks of age. Poult. Sci., 84: 1173-1178.
CrossRefPubMedDirect Link - Mashaly, M.M., G.L. Hendricks, M.A. Kalama, A.E. Gehad, A.O. Abbas and P.H. Patterson, 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci., 83: 889-894.
CrossRefPubMedDirect Link - Mazzi, C.M., J.A. Ferro, M.I.T. Ferro, V.J.M. Savino, A.A.D. Coelho and M. Macari, 2003. Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds. Genet. Mol. Biol., 26: 275-281.
CrossRefDirect Link - Munck, A., P.M. Guyre and N.J. Holbrook, 1984. Physiological functions of glucocorticoids in stress and their relation to pharmacological actions. Endocrine Rev., 5: 25-44.
CrossRefPubMedDirect Link - Nathan, D.B., E.D. Heller and M. Perek, 1976. The effect of short heat stress upon leucocyte count, plasma corticosterone level, plasma and leucocyte ascorbic acid content. Br. Poult. Sci., 17: 481-485.
PubMed - Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chickens 3. Temporal patterns of response. Poult. Sci., 79: 377-382.
CrossRefPubMedDirect Link - Tamzil, M.H., R.R. Noor, P.S. Hardjosworo, W. Manalu and C. Sumantri, 2013. [Polymorphism of the heat shock protein 70 gene in Kampong, Arabic and commercial chickens]. Jurnal Veteriner, 14: 317-326, (In Indonesian).
Direct Link - Tamzil, M.H., R.R. Noor, P.S. Hardjosworo, W. Manalu and C. Sumantri, 2013. Acute heat stress responses of three lines of chickens with different heat shock protein (HSP)-70 genotypes. Int. J. Poult. Sci., 12: 264-272.
CrossRefDirect Link - Toghyani, M., M. Shivazad, A.A. Gheisari and S.H. Zarkesh, 2006. Performance, carcass traits and hematological parameters of heat-stressed broiler chicks in response to dietary levels of chromium picolinate. Int. J. Poult. Sci., 5: 65-69.
CrossRefDirect Link - Zhen, F.S., H.L. Du, H.P. Xu, Q.B. Luo and X.Q. Zhang, 2006. Tissue and allelic-specific expression of Hsp70 gene in chickens: Basal and heat-stress-induced mRNA level quantified with real-time reverse transcriptase polymerase chain reaction. Br. Poult. Sci., 47: 449-455.
CrossRefDirect Link - Zulkifli, I., A. Al-Aqil, A.R. Omar, A.Q. Sazili and M.A. Rajion, 2009. Crating and heat stress influence blood parameters and heat shock protein 70 expression in broiler chickens showing short or long tonic immobility reactions. Poult. Sci., 88: 471-476.
CrossRefDirect Link