Hong Wang
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
Yanbin Li
Department of Biological and Agricultural Engineering,
University of Arkansas, Fayetteville, AR 72701, USA
Michael Slavik
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
ABSTRACT
Salmonella Typhimurium and Campylobacter jejuni are the most important bacterial pathogens associated with food borne diseases caused by consuming undercooked poultry or handling raw poultry and poultry products. Because of their low infectious dose of pathogens, a rapid, sensitive, simultaneous detection method is urgently needed. The objective of our research was to develop a sensitive biosensing method for rapid and simultaneous detection of S. Typhimurium and C. jejuni in chicken and ground turkey meat using magnetic nanobeads (MNBs) to capture and separate the target bacteria and quantum dots (QDs) to label the captured bacteria. In this research, both streptavidin conjugated QDs 530 and QDs 620 were coated with the specific biotin conjugated anti-S. Typhimurium and anti-C. jejuni antibodies, respectively. The MNBs were separately coated with the specific biotin conjugated anti-S. Typhimurium and anti-C. jejuni antibodies. The inoculated poultry samples were mixed with conjugated MNBs to capture the two target bacteria. After magnetic immunoseparation, the MNB-cell complexes were mixed with the conjugated QDs 530 and QDs 620 to form the MNB-cell-QD complexes. Unattached conjugated QDs were removed using magnetic separation. Finally, the fluorescence intensities of the MNB-S. Typhimurium-QD and MNB-C. jejuni-QD complexes were measured and correlated to the cell number of two target pathogens. The results showed that S. Typhimurium and C. jejuni in pure culture, chicken carcass and ground turkey wash solutions could be simultaneously separated and detected using the developed immunoassay. The fluorescence intensities at 530 and 620 nm wavelengths increased linearly with the increasing cell numbers of S. Typhimurium and C. jejuni, respectively. The assay detection limit was 30-50 cfu/ml and the assay time was less than 2 h.
PDF References Citation
How to cite this article
Hong Wang, Yanbin Li and Michael Slavik, 2014. Rapid and Simultaneous Detection of Salmonella and Campylobacter in Poultry Samples Using Quantum Dots Based Fluorescent Immunoassay Coupled with Magnetic Immunoseparation. International Journal of Poultry Science, 13: 611-618.
DOI: 10.3923/ijps.2014.611.618
URL: https://scialert.net/abstract/?doi=ijps.2014.611.618
DOI: 10.3923/ijps.2014.611.618
URL: https://scialert.net/abstract/?doi=ijps.2014.611.618
REFERENCES
- Baba, K. and K. Nishida, 2012. Single-molecule tracking in living cells using single quantum dot applications. Theranostics, 2: 655-667.
Direct Link - Bonjoch, X., L. Calvo, M. Soler, O. Ruiz-Rueda and L.J. Garcia-Gil, 2010. A new multiplexed real-time PCR assay to detect Campylobacter jejuni, C. coli, C. lari and C. upsaliensis. Food Anal. Methods, 3: 40-46.
CrossRefDirect Link - Chen, L. and J. Zhang, 2012. Bioconjugated magnetic nanoparticles for rapid capture of gram-positive bacteria. J. Biosens. Bioelectron. Vol. 11.
CrossRefDirect Link - Chinnathambi, S., S. Chen, S. Ganesan and N. Hanagata, 2014. Silicon quantum dots for biological applications. Adv. Healthcare Mater., 3: 10-29.
CrossRefDirect Link - Colombo, M., S. Carregal-Romero, M.F. Casula, L. Gutierrez and M.P. Morales et al., 2012. Biological applications of magnetic nanoparticles. Chem. Soc. Rev., 41: 4306-4334.
CrossRefDirect Link - Debretsion, A., T. Habtemariam, S. Wilson, D. Nganwa and T. Yehualaeshet, 2007. Real-time PCR assay for rapid detection and quantification of Campylobacter jejuni on chicken rinses from poultry processing plant. Mol. Cell. Prob., 21: 177-181.
CrossRefDirect Link - Fakruddin, M., K.S.B. Mannan and S. Andrews, 2013. Viable but nonculturable bacteria: Food safety and public health perspective. ISRN Microbiol., Vol. 2013.
CrossRefDirect Link - Galikowska, E., D. Kunikowska, E. Tokarska-Pietrzak, H. Dziadziuszko and J.M. Los et al., 2011. Specific detection of Salmonella enteric and Escherichia coli strains by using ELISA with bacteriophages as recognition agents. Eur. J. Clin. Microbiol. Infect. Dis., 30: 1067-1073.
CrossRefDirect Link - Goransson, J., T.Z.G. de la Torre, M. Stromberg, C. Russell, P. Svedlindh, M. Stromme and M. Nilsson, 2010. Sensitive detection of bacterial DNA by magnetic nanoparticles. Anal. Chem., 82: 9138-9140.
CrossRefDirect Link - Yaohua, H., W. Chengcheng, B. Bing, L. Mintong, R. Wang and Y. Li, 2014. Detection of Staphylococcus aureus using quantum dots as fluorescence labels. Int. J. Agric. Biol. Eng., 7: 77-83.
Direct Link - Jamieson, T., R. Bakhshi, D. Petrova, R. Pocock, M. Imani and A.M. Seifalian, 2007. Biological applications of quantum dots. Biomaterials, 28: 4717-4732.
CrossRefDirect Link - Jin, S., Y. Hu, Z. Gu, L. Liu and H.C. Wu, 2011. Application of quantum dots in biological imaging. J. Nanomater.
CrossRef - Jones, D.R., K.E. Anderson and J.Y. Guard, 2012. Prevalence of coliforms, Salmonella, Listeria and Campylobacter associated with eggs and the environment of conventional cage and free-range egg production. Poult. Sci., 91: 1195-1202.
CrossRefDirect Link - Kirsch, J., C. Siltanen, Q. Zhou, A. Revzin and A. Simonian, 2013. Biosensor technology: Recent advances in threat agent detection and medicine. Chem. Soc. Rev., 42: 8733-8768.
CrossRefDirect Link - LaGier, M.L., L.A. Joseph, T.V. Passaretti, K.A. Musser and N.M. Cirino, 2004. A real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli. Molecul. Cell. Prob., 18: 275-282.
CrossRefDirect Link - Leblanc-Maridor, M., F. Beaudeau, H. Seegers, M. Denis and C. Belloc, 2011. Rapid identification and quantification of Campylobacter coli and Campylobacter jejuni by real-time PCR in pure cultures and in complex samples. BMC Microbiol., Vol. 11.
CrossRefDirect Link - Lin, S., X. Wang, H. Zheng, Z. Mao, Y. Sun and B. Jiang, 2008. Direct detection of Campylobacter jejuni in human stool samples by real-time PCR. Can. J. Microbiol., 54: 742-747.
CrossRefDirect Link - Mayr, A.M., S. Lick, J. Bauer, D. Tharigen, U. Busch and I. Huber, 2010. Rapid detection and differentiation of Campylobacter jejuni, Campylobacter coli and Campylobacter lari in food, using multiplex real-time PCR. J. Food. Prot., 2: 241-250.
Direct Link - Melero, B., L. Cocolin, K. Rantsiou, I. Jaime and J. Rovira, 2011. Comparison between conventional and qPCR methods for enumerating Campylobacter jejuni in a poultry processing plant. Food Microbiol., 28: 1353-1358.
CrossRefDirect Link - Murphy, C., C. Carroll and K.N. Jordan, 2006. Environmental survival mechanisms of the foodborne pathogen Campylobacter jejuni. J. Applied Microbiol., 100: 623-632.
CrossRef - Oliver, J.D., 2005. The viable but nonculturable state in bacteria. J. Microbiol., 43: 93-100.
PubMedDirect Link - Oliver, J., M. Dagher and K. Linden, 2005. Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater. J. Water Health, 3: 249-257.
Direct Link - Pankhurst, Q.A., J. Connolly, S.K. Jones and J. Dobson, 2003. Applications of magnetic nanoparticles in biomedicine. J. Phys. D: Applied Phys., 36: R167-R181.
CrossRefDirect Link - Pires, S.M., H. Vigre, P. Makela and T. Hald, 2010. Using outbreak data for source attribution of human salmonellosis and campylobacteriosis in Europe. Foodborne Pathog. Dis., 7: 1351-1361.
CrossRef - Rosenthal, S.J., J.C. Chang, O. Kovtun, J.R. McBride and I.D. Tomlinson, 2011. Biocompatible quantum dots for biological applications. Chem. Biol., 18: 10-24.
CrossRefDirect Link - Samir, T.M., M.M. Mansour, S.C. Kazmierczak and H.M.E. Azzazy, 2012. Quantum dots: Heralding a brighter future for clinical diagnostics. Nanomedicine, 7: 1755-1769.
CrossRefDirect Link - Scallan, E., R.M. Hoekstra, F.J. Angulo, R.V. Tauxe and M.A. Widdowson et al., 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis., 17: 7-15.
CrossRefDirect Link - Tian, J., H. Zhao, M. Liu, Y. Chen and X. Quan, 2012. Detection of influenza A virus based on fluorescence resonance energy transfer from quantum dots to carbon nanotubes. Anal. Chem. Acta, 723: 83-87.
CrossRefPubMedDirect Link - Varshney, M. and Y. Li, 2007. Interdigitated array microelectrode based impedance biosensor coupled with magnetic nanoparticle-antibody conjugates for detection of Escherichia coli O157:H7 in food samples. Biosens. Bioelectron., 22: 2408-2414.
CrossRefDirect Link - Wang, H., Y. Li and M. Slavik, 2007. Rapid detection of listeria monocytogenes using quantum dots and nanobeads-based optical biosensor. J. Rapid Methods Automat. Microbiol., 15: 67-76.
CrossRef - Wang, H., Y. Li, A. Wang and M.F. Slavik, 2011. Rapid, sensitive and simultaneous detection of three foodborne pathogens using magnetic nanobead-based immunoseparation and quantum dot-based multiplex immunoassay. J. Food Protect®., 74: 2039-2047.
Direct Link - Wang, H., Y. Li and M. Slavik, 2014. Rapid detection of Campylobacter jejuni in poultry products using quantum dots and nanobeads based fluorescent immunoassay. Int. J. Poult. Sci., 13: 253-259.
CrossRefDirect Link - Wierucka, M. and M. Biziuk, 2014. Application of magnetic nanoparticles for magnetic solid-phase extraction in preparing biological, environmental and food samples. TrAC Trends Anal. Chem., 59: 50-58.
CrossRefDirect Link - Wu, S., N. Duan, Z. Shi, C. Fang and Z. Wang, 2014. Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labels. Anal. Chem., 86: 3100-3107.
CrossRefDirect Link - Xue, X., J. Pan, H. Xie, J. Wang and S. Zhang, 2009. Fluorescence detection of total count of Escherichia coli and Staphylococcus aureus on water-soluble CdSe quantum dots coupled with bacteria. Talanta, 77: 1808-1813.
CrossRefDirect Link - Yang, L. and Y. Li, 2006. Simultaneous detection of Escherichia coli O157∶H7 and Salmonella Typhimurium using quantum dots as fluorescence labels. Analyst, 131: 394-401.
CrossRefDirect Link - Yoo, J.H., D.H. Woo, M.S. Chang and M.S. Chun, 2014. Microfluidic based biosensing for Escherichia coli detection by embedding antimicrobial peptide-labeled beads. Sensors Actuators B: Chem., 191: 211-218.
CrossRefDirect Link - Zeng, Q., Y. Zhang, K. Song, X. Kong, M.C.G. Aalders and H. Zhang, 2009. Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through flexible coupling between quantum dots and antibody. Talanta, 80: 307-312.
CrossRefDirect Link