Sandra G. Velleman
Department of Animal Sciences, Ohio Agricultural Research and Development Center, Ohio State University, 1680 Madison Ave, Wooster, OH-44691, USA
ABSTRACT
The effect of growth selection in turkeys was studied for its effect on the expression of adipogenic genes in the pectoralis major (p. major) muscle both in vivo and in vitro in a random bred control line (RBC2) and a subline F selected long-term from the RBC2 for only increased 16-wk body weight. The expression of the adipogenic transcription factors peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein α (C/EBPα) and stearoyl-CoA desaturase (SCD) were measured in p. major muscle tissue isolated from 18 and 24 d old embryos and 1 and 16-wk old RBC2 and F line turkeys and in vitro with adult myoblasts (satellite cells) isolated from RBC2 and F line turkey p. major muscle tissue during proliferation and differentiation. Both in vivo and in vitro, the F line expressed significantly higher levels of PPARγ, C/EBPα and SCD. The data from the current study provides novel information about the effect of growth selection in turkeys on the potential of fat formation in vivo in the p. major muscle and the possible transdifferentiation of myogenic satellite cells to an adipogenic lineage.
PDF References Citation
How to cite this article
Sandra G. Velleman, 2014. Effect of Growth Selection on Adipogenic Gene Expression During the Development of the Turkey Breast Muscle. International Journal of Poultry Science, 13: 680-684.
DOI: 10.3923/ijps.2014.680.684
URL: https://scialert.net/abstract/?doi=ijps.2014.680.684
DOI: 10.3923/ijps.2014.680.684
URL: https://scialert.net/abstract/?doi=ijps.2014.680.684
REFERENCES
- Asakura, A., M.A. Rudnicki and M. Komaki, 2001. Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic and adipogenic differentiation. Differentiation, 68: 245-253.
CrossRefDirect Link - Halevy, O., A. Geyra, M. Barak, Z. Uni and D. Sklan, 2000. Early posthatch starvation decreases satellite cell proliferation and skeletal muscle growth in chicks. J. Nutr., 130: 858-864.
PubMedDirect Link - Hu, E., P. Tontonoz and B.M. Spiegelman, 1995. Transdifferentiation of myoblasts by the adipogenic transcription factors PPAR gamma and C/EBP alpha. Proc. Natl. Acad. Sci., 92: 9856-9860.
PubMedDirect Link - Lilburn, M.S. and K.E. Nestor, 1991. Body weight and carcass development in different lines of Turkeys. Poult. Sci., 70: 2223-2231.
CrossRefDirect Link - Mauro, A., 1961. Satellite cell of skeletal muscle fibers. J. Biophys. Biochem. Cytol., 9: 493-495.
PubMedDirect Link - Moss, F.P. and C.P. Leblond, 1971. Satellite cells as the source of nuclei in muscles of growing rats. Anatomical Record, 170: 421-435.
CrossRefDirect Link - Mozdziak, P.E., T.J. Walsh and D.W. McCoy, 2002. The effect of early posthatch nutrition on satellite cell mitotic activity. Poult. Sci., 81: 1703-1708.
CrossRefPubMedDirect Link - Nestor, K.E., 1977. Genetics of growth and reproduction in the Turkey 5. Selection for increased body weight alone and in combination with increased egg production. Poult. Sci., 56: 337-347.
CrossRefDirect Link - Shefer, G., M. Wleklinski-Lee and Z. Yablonka-Reuveni, 2004. Skeletal muscle satellite cells can spontaneously enter an alternative mesenchymal pathway. J. Cell Sci., 117: 5393-5404.
Direct Link - Velleman, S.G., C.S. Coy and D.A. Emmerson, 2014. Effect of the timing of posthatch feed restrictions on broiler breast muscle development and muscle transcriptional regulatory factor gene expression. Poult. Sci., 93: 1484-1494.
CrossRefDirect Link - Velleman, S.G., C.S. Coy and D.A. Emmerson, 2014. Effect of the timing of posthatch feed restrictions on the deposition of fat during broiler breast muscle development. Poult. Sci., 93: 2622-2627.
CrossRefDirect Link - Velleman, S.G., K.E. Nestor, C.S. Coy, I. Harford and N.B. Anthony, 2010. Effect of posthatch feed restriction on broiler breast muscle development and muscle transcriptional regulatory factor gene and heparan sulfate proteoglycan expression. Int. J. Poult. Sci., 9: 417-425.
CrossRefDirect Link - Velleman, S.G., X. Liu, K.E. Nestor and D.C. McFarland, 2000. Heterogeneity in growth and differentiation characteristics in male and female satellite cells isolated from Turkey lines with different growth rates. Comp. Biochem. Physiol. Part A: Mol. Integrat. Physiol., 125: 503-509.
CrossRefDirect Link - Yu, Y.H., B.H. Liu, H.J. Mersmann and S.T. Ding, 2006. Porcine peroxisome proliferator-activated receptor γ induces transdifferentiation of myocytes into adipocytes. J. Anim. Sci., 84: 2655-2665.
CrossRefDirect Link