Muhammad Shah Zaman
Department of Plant Breeding and Genetics, NWFP Agricultural University Peshawar, Pakistan
Azra Quraishi
Department of Plant Breeding and Genetics, NWFP Agricultural University Peshawar, Pakistan
Ghulam Hassan
Department of Plant Breeding and Genetics, NWFP Agricultural University Peshawar, Pakistan
Raziuddin .
Department of Plant Breeding and Genetics, NWFP Agricultural University Peshawar, Pakistan
Sardar Ali
Not Available
Abdul Khabir
Not Available
Naheed Gul
Not Available
ABSTRACT
The effect of three different auxins viz, naphthalene acetic acid (NAA), indole acetic acid (IAA) and indole butyric acid (IBA) each at four levels (0, 0.1, 0.5 and 1.0 mg l-1) was evaluated on meristem culture of Potato (Solanum tuberosum L.) for production of virus-free plantlets. Maximum plantlet height (8.3 cm), largest number of nodes/plantlet (7.3) and highest number of leaves/plantlet (8.9) were recorded at 0.5 mg l-1 of NAA followed by IBA at 1 mg l-1, whereas extensive number of roots/plantlet (23.7) as well as the earliest microtuber formation (17 days, after transplantation) were recorded at 1 mg l-1 of IBA followed by NAA at 0.1 mg l-1. Largest root (4.2 cm) was observed for IAA at 1 mg l-1 concentration. Enzyme Linked Immunosorbent Assay (ELISA) test proved that all plants were free of Potato Virus X (PVX), Potato Virus Y (PVY) and Potato Leaf Role Virus (PLRV), except one which was positive for Potato Virus X (PVX) virus.
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How to cite this article
Muhammad Shah Zaman, Azra Quraishi, Ghulam Hassan, Raziuddin ., Sardar Ali, Abdul Khabir and Naheed Gul, 2001. Meristem Culture of Potato (Solanum tuberosum L.) for Production of Virus-Free
Plantlets. Journal of Biological Sciences, 1: 898-899.
DOI: 10.3923/jbs.2001.898.899
URL: https://scialert.net/abstract/?doi=jbs.2001.898.899
DOI: 10.3923/jbs.2001.898.899
URL: https://scialert.net/abstract/?doi=jbs.2001.898.899
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