Abdul Haleem Shah
Gomal University, Dera Ismail Khan, NWFP, Pakistan
Abdul Hameed
Department of Biological Science, Ouaid-1-Azam University, Islamabad, Pakistan
Safia Ahmad
Department of Biological Science, Ouaid-1-Azam University, Islamabad, Pakistan
Gul Majid Khan
Department of Pharmaceutics, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, NWFP, Pakistan
ABSTRACT
The objective of this study was to improve the L-lysine production by Corynebacterium glutamicum. For this purpose culture conditions for the lysine fermentation by the bacteria were optimized. In this regard the role of various physical and nutritional parameters was examined. The culture was incubated in a 500 ml Erlenmeyer flask in a rotatory shaking incubator at 200 rpm. The appropriate conditions were obtained only when 50 ml medium was charged for fermentation at 30 oC, pH 7.5 and at 10% inoculum size. The finally selected medium per 100 ml distilled water formulated was 10 g glucose, 2.5 g ammonium sulfate, 2.0 g calcium carbonate, 0.5 g bactocasamino acid, 20 μg thiamine hydrochlorid, 5 μg D-biotin, 0.1 g potassium dihydrogen phosphate, 0.05 g magnesium sulfate heptahydrate, 0.2 mg ferrous sulfate heptahydrate and 0.2 mg manganese chloride tetrahydrate. The optimized culture conditions resulted into elevated amount of L-lysine.
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How to cite this article
Abdul Haleem Shah, Abdul Hameed, Safia Ahmad and Gul Majid Khan, 2002. Optimization of Culture Conditions for L-Lysine Fermentation by Corynebacterium
glutamicum. Journal of Biological Sciences, 2: 151-156.
DOI: 10.3923/jbs.2002.151.156
URL: https://scialert.net/abstract/?doi=jbs.2002.151.156
DOI: 10.3923/jbs.2002.151.156
URL: https://scialert.net/abstract/?doi=jbs.2002.151.156