Masood Akhtar
lmmunoparasitology Laboratory, Department of Veterinary Parasitology
M. Mazhar Ayaz
lmmunoparasitology Laboratory, Department of Veterinary Parasitology
C.S. Hayat
lmmunoparasitology Laboratory, Department of Veterinary Parasitology
Abdul Jabbar
lmmunoparasitology Laboratory, Department of Veterinary Parasitology
M. A. Hafeez
Department of Veterinary Microbiology, University of Agriculture, Faisalabad, Pakistan
M. Ashfaque
Department of Veterinary Microbiology, University of Agriculture, Faisalabad, Pakistan
I. Hussain
Department of Veterinary Microbiology, University of Agriculture, Faisalabad, Pakistan
ABSTRACT
The involvement of secretary immunoglobulin system in the protection of mucous surfaces by locally produced antibodies lead to the demonstration of IgA. IgA in the intestine of immune birds may limit coccidian replication. Enzyme linked immunospot (ELISPOT) assay was employed to detect the mucosal immune response (IgA antibody secreting splenic cells) in chicken vaccinated with egg adapted gametocytes antigen. Twenty to twenty-five % (200,000 to 250,000/106 cells) of chicken splenic cells stimulated with gametocytes antigen were detected as IgA secretors. Results of the ELISPOT revealed that spleen is one of the major source of cells producing IgA in chickens. Such a high number of IgA antibody secreting cells indicate protection in birds vaccinated with gametocytes antigen via oral route against coccidiosis. ELISPOT assay is found to be an advantageous alternative to conventional haemolytic plaque technique, being simpler and of equivalent or even greater sensitivity.
PDF References Citation
How to cite this article
Masood Akhtar, M. Mazhar Ayaz, C.S. Hayat, Abdul Jabbar, M. A. Hafeez, M. Ashfaque and I. Hussain, 2002. ELISPOT Assay for Detection of IgA Secreting Splenic Cells in Chicken. Journal of Biological Sciences, 2: 554-555.
DOI: 10.3923/jbs.2002.554.555
URL: https://scialert.net/abstract/?doi=jbs.2002.554.555
DOI: 10.3923/jbs.2002.554.555
URL: https://scialert.net/abstract/?doi=jbs.2002.554.555
REFERENCES
- Akhtar, M., M.M. Ayaz, C.S. Hayat, M. Ashfaque and I. Hussain, 2002. Adaptation of Eimeria tenella (local isolate) sporozoites in chicken embryos. Pak. Vet. J., 22: 40-41.
Direct Link - Czerkinsky, C., L.A. Nilsson, A. Tarkowski, W.J. Koopman, J. Mestecky and O. Ouchterlony, 1988. The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting-Cells: Methodology and Applications. In: Theoretical and Technical Aspects of ELISA and Other Solid Phase Immunoassays, Kemeny, D.M. and S.J. Challacombe (Eds.). John Wiley and Sons, Oxford.