Abdulrahman Khazim Al Asmari
Research Center, Armed Forces Hospital, Riyadh, Saudi Arabia
ABSTRACT
The present study was conducted to investigate the inflammatory response induced by Echis pyramidum venom (EpV) in the rat hind-paw by measuring paw edema. Non-heated EpV (3.125-50 µg paw-1) caused a marked paw edema (plateau) accompanied by intense haemorrhage(not shown); whereas heated venom (97°C, 30 s; 3.125-50 µg paw-1) produced a dose and time-dependent non-haemorrhagic edema. The response with heated EpV was maximal within 15 min, disappearing over 24 h. Heated EpV (25 µg paw-1) was optimized to test the effect of various drugs on edema induced by the EpV. The results showed that dexamethasone, H1 and H3 receptor antagonist cyproheptadine and H1 receptor antagonist chlorpheniramine markedly (P<0.001) reduced the EpV-induced rat paw edema, whereas the cyclooxygenase inhibitor diclofenac and the proteinase inhibitor (aprotinin) produced a moderately significant (P<0.01) inhibition of edema. The commercially available antivenom was found to be ineffective when administered intravenously, whereas local administration by coupling the EpV and antivenom had a highly significant (P<0.001) effect. We conclude that EpV induced edema; while the expected principal mediators of this inflammatory response were serotonin, histamine, cyclo-oxygenase, other prostaglandins (PGs) and cytokines. Finally, the antivenom given intravenously failed to reduce edema.
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How to cite this article
Abdulrahman Khazim Al Asmari, 2003. Pharmacological Characterization
of the Rat Paw Edema Induced by Echis pyramidum Venom. Journal of Biological Sciences, 3: 824-833.
DOI: 10.3923/jbs.2003.824.833
URL: https://scialert.net/abstract/?doi=jbs.2003.824.833
DOI: 10.3923/jbs.2003.824.833
URL: https://scialert.net/abstract/?doi=jbs.2003.824.833
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