M.A. Rahman
Department of Horticulture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
C.C. Ainsworth
Plant Molecular Biology Laboratory, Imperial College,
Wye Campus, Wye, Ashford, Kent TN25 SAH, England
ABSTRACT
Sorrel (Rumex acetosa L.) is dioecious model plant with heteromorphic sex chromosome system with 2n=12+XX in females and 2n=12+XY1Y2 in males. Sex is determined by the X:autosome balance. The major sex determining genes locate on X-chromosomes and the Y-chromosomes are not required for sex expression. Y-chromosomes, which contain genes for pollen fertility, constitute 26% of male genome but the structure and functions of the Ys are largely unknown. AFLP using MseI/PstI digestion reveals very low levels of male specific markers in R. acetosa and related species. Fifteen primer-combinations tested in R. acetosa generated 1085 bands, 4 of which were male specific. Two male associated DNA fragments, MADR1 (583 bp) and MADR2 (133 bp), were isolated and sequenced. These sequences show similarity to short stretches (up to 20-30 bp) of known sequences from several organisms. The deduced amino acid sequence of MADR1 showed similarity to phosphatase genes from Arabidopsis thaliana, while MADR2 showed similarity to retroelement sequences. The generation of very few male specific AFLP markers and their sequence similarity to the stretches of human DNA from autosomes and both the X and Y-chromosomes indicate the likely origin of the Ys from the X-chromosome.
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How to cite this article
M.A. Rahman and C.C. Ainsworth, 2004. AFLP Analysis of Genome Difference Between Male and Females in Dioecious Plant Rumex acetosa. Journal of Biological Sciences, 4: 160-169.
DOI: 10.3923/jbs.2004.160.169
URL: https://scialert.net/abstract/?doi=jbs.2004.160.169
DOI: 10.3923/jbs.2004.160.169
URL: https://scialert.net/abstract/?doi=jbs.2004.160.169
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