N.A. Siddique
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
M.A. Bari
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
Sharmin Shahnewaz
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
M.H. Rahman
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
M.R. Hasan
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
M.S.I.
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
LiveDNA: Khan
M.S. Islam
Institute of Biological Sciences, University ofRajshahi, Rajshahi-6205, Bangladesh
ABSTRACT
Callus cultures were initiated from nodal segments on Murashige and Skoog[19] medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
PDF References
How to cite this article
N.A. Siddique, M.A. Bari, Sharmin Shahnewaz, M.H. Rahman, M.R. Hasan, M.S.I. and M.S. Islam, 2004. Plant Regeneration of Withania somnifera (L.) Dunal
(Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant
in Bangladesh. Journal of Biological Sciences, 4: 219-223.
DOI: 10.3923/jbs.2004.219.223
URL: https://scialert.net/abstract/?doi=jbs.2004.219.223
DOI: 10.3923/jbs.2004.219.223
URL: https://scialert.net/abstract/?doi=jbs.2004.219.223
REFERENCES
- Suffness, M. and J. Douros, 1982. Current status of the NCI plant and animal product program. J. Nat. Prod., 45: 1-14.
CrossRef - Roja, G., M.R. Heble and A.T. Sipahimalani, 1991. Tissue cultures of Withania somnifera: Morphogenesis and withanolide synthesis. Phytother. Res., 5: 185-187.
CrossRefDirect Link - Murch, S.J., K.L. Choffe, J.M.R. Victor, T.Y. Slimmon, S.K. Raj and P.K. Saxena, 2000. Thidiazuron-induced plant regeneration from hypocotyl cultures of St. John's wort (Hypericum perforatum. cv Anthos). Plant Cell Rep., 19: 576-581.
CrossRefDirect Link - Rani, G. and I.S. Grover, 1999. In vitro callus induction and regeneration studies in Withania somnifera. Plant Cell Tissue Organ. Cult., 57: 23-27.
CrossRef - Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Fauconnier, M.L., M. Jaziri, J. Homes, K Shirnomura and M. Marlier, 1996. Anthemis nabilis L. (Roman Chamomile) in vitro Culture, Micropropagation and the Production of Essential Oils. In: Biotechnology in Agriculture and Foresty, Bajaj, Y.P.S. (Ed.). Vol. 37, Springer, Berlin Heidelberg, pp: 16-37.
- Cuenca, S., J.B.A. Marco and R. Parra, 1999. Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae). Plant Cell Rep., 18: 674-679.
CrossRefDirect Link