Masudur Rahman Gazi
Department of Applied Biological Science, Saga University, Saga 840-8502, Japan
K. Kanda
Department of Applied Biological Science, Saga University, Saga 840-8502, Japan
F. Kato
Department of Applied Biological Science, Saga University, Saga 840-8502, Japan
ABSTRACT
The objective of this study was to optimise the cultural factors to enhance the production of antioxidant from Saccharomyces cerevisiae IFO 2373 (IFO 2373). Carbon and nitrogen sources of the medium were optimised along with other cultural conditions for growth and antioxidant activity generation. The antioxidant activity of the cell free extract was detected by measuring the free radical scavenging activity. Other than poly saccharide (water soluble starch), mono and di saccharide sources were observed solely responsible for antioxidant activity generation. The culture broth had higher antioxidant activity (202 u mL ha-1 ) when sucrose was used as carbon source. Polypeptone containing medium was found better for antioxidant activity generation among the tested organic and inorganic nitrogen sources. The highest antioxidant activity (296 u mL ha-1 ) was achieved at 30°C with 0.7 L min ha-1 of aeration rate. These results are promising because we succeeded in increasing the antioxidant activity almost 2.3 fold over the initial production obtained from half YPD (H-YPD) culture broth.
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How to cite this article
Masudur Rahman Gazi, K. Kanda and F. Kato, 2004. Optimisation of Various Cultural Conditions on Growth and Antioxidant Activity Generation by Saccharomyces cerevisiae IFO 2373. Journal of Biological Sciences, 4: 224-228.
DOI: 10.3923/jbs.2004.224.228
URL: https://scialert.net/abstract/?doi=jbs.2004.224.228
DOI: 10.3923/jbs.2004.224.228
URL: https://scialert.net/abstract/?doi=jbs.2004.224.228
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