H. C.C. Maduka
Department of Biochemistry, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria
Z. S.C. Okoye
Department of Biochemistry, Faculty of Medical Sciences, University of Jos, Jos, Nigeria
M. Mahmood
Department of Biochemistry, Faculty of Medical Sciences, University of Jos, Jos, Nigeria
ABSTRACT
The amino acid profile and anionic composition of three aqueous preparations of Sacoglottis gabonensis stem bark were determined. Curde extract of Sacoglottis gabonensis stem bark prepared in 4% ethanol (1:10w/v), the deffated fraction of the ethanol crude extract and methanol extract (1:10w/v) were analyzed. The anionic composition analysis showed that in ethanolic extract, Cl- was present in highest amount followed by NO3- then F- and lastly SO42- being 27.7 ± 1.25, 6.82 ± 0.11, 5.10 ± 0.30 and 3.08 ± 0.11 ppm respectively. The deffated ethanol extract contained Cl- in highest quantity followed by SO42- and then NO3- and finally F-being 24.8 ± 0.98, 12.34 ± 0.15, 9.14 ± 0.38 and 5.10 ± 0.30 ppm respectively. The methanolic extract contained Cl-, SO42-, NO3- and F- to the extent of 30.3 ± 1.08, 6.77 ± 0.11, 4.77 ± 0.20 and 4.12 ± 0.18 ppm respectively of the anions. Results showed that while the ethanol crude extract contained eleven amino acids including seven essential amino acids (1 ys, Leu, Ile, Tyr, Thr, His and Val), the defatted extract and methanolic extract contained 13 and 17 amino acids respectively including all the nine essential amino acids. Both the defatted portion and methanolic extract contained Cys thus suggesting a role in detoxification of autooxidizable drugs. The bark extract can serve as alternative source of essential amino acids and hence relevant in nutrition. Toxic species like CN, Cd and Hg that could inhibit drug metabolizing enzymes were absent. Methanol is the best extractor while defatting unmasked Phe and Met which were not detected in the crude extract.
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How to cite this article
H. C.C. Maduka, Z. S.C. Okoye and M. Mahmood, 2004. Amino Acids and Anionic Components of Sacoglottis gabonenis Stem Bark Extract, A Nigerian Alcoholic Beverage Additive. Journal of Biological Sciences, 4: 55-61.
DOI: 10.3923/jbs.2004.55.61
URL: https://scialert.net/abstract/?doi=jbs.2004.55.61
DOI: 10.3923/jbs.2004.55.61
URL: https://scialert.net/abstract/?doi=jbs.2004.55.61
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