Renuka Malhotra
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India, 143-005
Balwant Singh
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India, 143-005
ABSTRACT
Total lipids of Saccharomyces cerevisiae were isolated by chloroform and methanol (2:1). Glycolipids were separated from total lipids by silicic acid chromatography. Glycolipid’s constituent sugars and fatty acids were analyzed by using Gas Liquid Chromatography. Galactose was the prominent sugar followed by mannose. Relative concentrations of fucose, mannose, galactose and glucose in the glycolipid were 5.3, 35.2, 55.1 and 4.2%. 16:0, 18:0, 18:1, 18:2 and 18:3 were the major fatty acids of the total glycolipids. Oleic acid was the dominating fatty acid followed by linoliec acid. They were separated into different fractions by using DEAE-Sephadex ion exchange chromatography. Glycolipids were fractionated and identified as cerebrosides, ceramide polyhexosides, sulfatides, monoglucosyldiglycerides and diglucosyldiglycerides. Ceramide polyhexosides were present in higher concentration as compared to other fractions.
PDF References Citation
How to cite this article
Renuka Malhotra and Balwant Singh, 2005. Glycolipids of Saccharomyces cerevisiae Cell. Journal of Biological Sciences, 5: 253-256.
DOI: 10.3923/jbs.2005.253.256
URL: https://scialert.net/abstract/?doi=jbs.2005.253.256
DOI: 10.3923/jbs.2005.253.256
URL: https://scialert.net/abstract/?doi=jbs.2005.253.256
REFERENCES
- Hannun, Y.A. and C. Luberto, 2000. Ceramide in the Eukaryotic stress response. Trends Cell Biol., 10: 73-80.
Direct Link - Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
CrossRefPubMedDirect Link - Roe, J.H., 1955. The determination of sugar in blood and spinal fluid with anthrone reagent. J. Biol. Chem., 212: 335-343.
Direct Link - Gupta, S., S.C. Sharma and B. Singh, 1994. Changes in composition and peroxidation of yeast membrane lipids during ethanol stress. Acta Microbiol. Immunol. Hung, 41: 197-204.
Direct Link - Wu, J., D.M. Seliskar and J.R. Gallagher, 1998. Stress tolerance in the marsh plant Spartina patens: Impact of NaCl on growth and root plasma membrane lipid composition. Physiologia Plantarum, 102: 307-317.
CrossRefDirect Link