Abbas Rezaee
Department of Environmental Health, Faculty of Medical Sciences,
Tarbiat Modares University, Tehran, Iran
Majid Ansari
Department of Environmental Health, Faculty of Medical Sciences,
Tarbiat Modares University, Tehran, Iran
Fathemeh Zandi
Department of Environmental Health, Faculty of Medical Sciences,
Tarbiat Modares University, Tehran, Iran
ABSTRACT
Shuttle vector is a DNA molecule (e.g. plasmid) that is able to replicate in two different host organisms and can therefore be used to shuttle or convey genes from one to the other. In this study, a new shuttle vector was constructed that can replicate autonomously in Acetobacter xylinum and Escherichia coli. The new plasmid shuttle vector, designated pTMU30, was constructed from E. coli plasmid pUC18 and origin of replication (ori) of a cryptic plasmid pTMU20, which was originally isolated by us from a naturally occurring cellulose producing Acetobacter sp. The resulting shuttle vector, harboured resistance gene for ampicillin and capable of replicating in Acetobacter xylinum and E. coli. Transformation and replication of shuttle vector confirmed with antibiotic resistant experiments in the transformants bactrium. The vector was successfully transformed in Acetobacter xylinum and E. coli. This shuttle vector might used as a basis for genetic manipulation studies of Acetobacter xylinum.
PDF References Citation
How to cite this article
Abbas Rezaee, Majid Ansari and Fathemeh Zandi, 2005. Construction of a New Shuttle Vector for Acetobacter xylinum and Escherichia coli. Journal of Biological Sciences, 5: 274-278.
DOI: 10.3923/jbs.2005.274.278
URL: https://scialert.net/abstract/?doi=jbs.2005.274.278
DOI: 10.3923/jbs.2005.274.278
URL: https://scialert.net/abstract/?doi=jbs.2005.274.278
REFERENCES
- Saxena, I.M., K. Kudlicka and R.M. Brown, 1994. Characterization of genes in the cellulose synthesizing operon (acs operon) of Acetobacter xylinum implications for cellulose crystallization. J. Bacteriol., 176: 5735-5752.
PubMed - Lynd, L.R., P.J. Weimer, W.H. van Zyl and I.S. Pretorius, 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev., 66: 506-577.
CrossRefPubMedDirect Link - Yanisch-Perron, C., J. Vieira and J. Messing, 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33: 103-119.
PubMed - Birnboim, H.C. and J. Doly, 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res., 7: 1513-1523.
CrossRefPubMedDirect Link