M. M. Rahman
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
M. N. Amin
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
T. Ahamed
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
S. Ahmad
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
A. Habib
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh
R. Ahmed
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
M. B. Ahmed
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
M. R. Ali
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh
ABSTRACT
An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.
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How to cite this article
M. M. Rahman, M. N. Amin, T. Ahamed, S. Ahmad, A. Habib, R. Ahmed, M. B. Ahmed and M. R. Ali, 2005. In vitro Rapid Propagation of Black Thorn (Kaempferia galanga L.): A Rare Medicinal and Aromatic Plant of Bangladesh. Journal of Biological Sciences, 5: 300-304.
DOI: 10.3923/jbs.2005.300.304
URL: https://scialert.net/abstract/?doi=jbs.2005.300.304
DOI: 10.3923/jbs.2005.300.304
URL: https://scialert.net/abstract/?doi=jbs.2005.300.304
REFERENCES
- Wong, K.C., K.S. Ong and C.L. Lim, 1992. Compositon of the essential oil of rhizomes of kaempferia galanga L. Flavour Fragrance J., 7: 263-266.
CrossRefDirect Link - Malamug, J.J.F., H. Inden and T. Asahira, 1991. Plantlet regeneration and propagation from ginger callus. Scient. Hortic., 48: 89-97.
Direct Link - Kackar, A., S.R. Bhat, K.P.S. Chandel and S.K. Malik, 1993. Plant regeneration via somatic embryogenesis in ginger. Plant Cell Tissue Organ Cult., 32: 289-292.
CrossRefDirect Link - Balachandran, S.M., S.R. Bhat and K.P.S. Chandel, 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rose.). Plant Cell Rep., 8: 521-524.
CrossRefDirect Link - Rahman, M.M., M.N. Amin, H.S. Jahan and R. Ahmed, 2004. In vitro regeneration of plantlets of Curcuma longa Linn. A valuable spice plant in Bangladesh. Asian J. Plant Sci., 3: 306-309.
CrossRefDirect Link - Rahman, M.M., M.N. Amin, T. Ahamed, M.R. Ali and A. Habib, 2004. Efficient plant regeneration through somatic embryogenesis from leaf base-derived callus of Kaempferia galanga L. Asian J. Plant Sci., 3: 675-678.
CrossRefDirect Link - Inden, H., T. Asahira and A. Hirano, 1988. Micropropagation of ginger. Acta Hortic., 230: 177-184.
CrossRefDirect Link