Mohamed Abdulrahman Al-Omar
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Mohammed Nasir Al-Arifi
Director of Drug and Poison Information Center, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
ABSTRACT
Scopoletin, 7-hydroxy-5-methoxycoumarin, has been found to be a moderate inhibitor for aldehyde oxidase, a molybdo-flavoenzymes. It inhibits the release of superoxide anion more efficiently than hydrogen peroxide or substrate oxidation. Statistically, the inhibition of three-specific aldehyde oxidase substrates was significant (p<0.005) in the presence of 100 μM Scopoletin. A progressive and non-competitive inhibition has been observed with inhibitor constant (Ki) value of 57.2 ±3.3 μM using indole-3-aldehyde and oxygen as a substrate and electron acceptor, respectively. A specific site for interaction between Scopoletin and aldehyde oxidase has been proposed and its effect on the reactive oxygen species production has been discussed. The effect of Scopoletin on aldehyde oxidase activity has been compared with that of traditional and specific inhibitors, such as menadione.
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How to cite this article
Mohamed Abdulrahman Al-Omar and Mohammed Nasir Al-Arifi, 2005. Comparative Effects of Scopoletin and Menadione on Aldehyde Oxidase Activity of Guinea Pig Liver. Journal of Biological Sciences, 5: 525-531.
DOI: 10.3923/jbs.2005.525.531
URL: https://scialert.net/abstract/?doi=jbs.2005.525.531
DOI: 10.3923/jbs.2005.525.531
URL: https://scialert.net/abstract/?doi=jbs.2005.525.531
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CrossRefDirect Link - Al-Omar, M.A., C. Beedham, F. Belal, J.A. Smith and A.A. El-Emam, 2005. Fluorimetric measurement of hydrogen peroxide produced during aldehyde oxidase catalysed oxidation using scopoletin. J. Med. Sci., 5: 10-20.
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CrossRefPubMedDirect Link - Al-Omar, M.A., C. Beedham and I.A. Al-Sarra, 2004. Pathological roles of reactive oxygen species and their defence mechanisms. Saudi Pharm. J., 12: 1-18.
Direct Link - Al-Omar, M., 2005. Effect of 2-hydroxy-1,4-naphthaquinone, a natural dye of Henna, on aldehyde oxidase activity in guinea pig liver. J. Med. Sci., 5: 163-168.
Direct Link - Al-Omar, M.A., H.I. El-Subbagh, C. Beedham and J.A. Smith, 2005. Role of molybdenum hydroxylases in diseases. Saudi. Pharm. J., 13: 1-13.
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